Pyruvate Kinase, Tumor Type M2, Recombinant, Human

MTAP (Methylthioadenosine phosphorylase) is important in polyamine metabolism and for the salvage of both adenine and methionine. It is involved in the metabolism of MeSAdo (methylthioadenosine), a by-product of the synthesis of the polyamines spermidine and spermine. MTAP is deficient in many cancers because the MTAP gene is co-deleted with the tumor suppressor p16. Source: Recombinant human PKM2, full length, with N-terminal His tag, expressed in E. coli. Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. , Enzyme Activity: , A 50ul PKM2 reaction is conducted in a buffer containing 50mM Tris, pH 7.4, 10mM MgCl2, 50mM KCl, 2mM ADP, 10mM phosphoenolpyruvate (PEP) at RT for 15 minutes. ATP production is detected using Kinase-Glo(R) Luminescent Kinase Assay Platform. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.