Item number | Size | Datasheet | Manual | SDS | Delivery time | Quantity | Price |
---|---|---|---|---|---|---|---|
P4072.100 | 100 U | - | - |
3 - 19 business days* |
379.00€
|
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Venom exonuclease (Phosphodiesterase I) successively hydrolyzes 5'-mononucleotides from 3'-hydroxy-terminated ribo-and deoxyribo-oligonucleotides. The enzyme has been widely utilized as a tool for structural and sequence studies of nucleic acids. The enzyme has been purified with endonuclease activity being eliminated as well as 5'-nucleotidase and nonspecific monophosphatase. It is nonspecific with respect to base or sugar moieties of nucleotides. A variety of synthetic substrates are hydrolyzed. The exonuclease will not recognize nucleoside units in the syn conformation. ADP-ribosylated proteins are cleaved at the pyrophosphate linkages by venom phosphodiesterase to yield phosphoribosyl-AMP. Activity: >20 units per mg dry weight, Absorbance (A280): As reported, Unit Definition: 1 unit hydrolyzes 1 umole of p-nitrophenyl thymidine-5-phosphate per minute at 25ºC, pH 8.9. Nucleotidase: <8%, Optimum pH: 9.8-10.4, Inhibitors: Reducing agents such as glutathione, cysteine and ascorbic acid. It is completely inhibited by 5mM EDTA while ATP, ADP and AMP are partial inhibitors. Activators: The enzyme has an absolute requirement for Mg2+ indicates an optimum concentration of 15mM. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Keywords: | Venom phosphodiesterase 1 |
Supplier: | United States Biological |
Supplier-Nr: | P4072 |
Properties
Conjugate: | No |
Format: | Molecular Biology Grade |
Database Information
CAS : | 9025-82-5| Matching products |
UniProt ID : | J3SEZ3 | Matching products |
Handling & Safety
Storage: | -20°C |
Shipping: | +4°C (International: +4°C) |
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