NF-kappaB (p65) (human, recombinant)

NF-kappaB p65 is a ubiquitously expressed transcription factor that is a subunit of the NF-kappaB complex and is encoded by the RELA gene in humans. It is composed of an N-terminal Rel homology domain, which mediates dimerization, nuclear localization, and DNA and protein interactions, and two C-terminal transactivation domains that are subject to a variety of post-translational modifications and regulate the transcriptional activity of p65. NF-kappaB p65 regulates the expression of a large number of genes in response to inflammatory and environmental cues that play critical roles in innate and adaptive immunity and cellular differentiation. Genome-wide deletion of Rela in mice is embryonic lethal. Silencing of Rela induces tumor cell apoptosis in a murine Lewis lung carcinoma model, and RELA silencing in THP-1 monocytes decreases secreted levels of IL-1beta and TNF-alpha induced by LPS. NF-kappaB p65 is overexpressed in the inflamed joints of patients with rheumatoid arthritis and naïve CD4 T cells isolated from the whole blood of patients with multiple sclerosis have increased phosphorylation of NF-kappaB p65. Cayman's NF-kappaB (p65) (human, recombinant) protein can be used for Western blot and ELISA applications.Synonyms: NF-kappaB3, Nuclear Factor-kappaB (p65). Source: Recombinant N-terminal histidine-tagged p65 (386-480) expressed in E. coli. Amino Acids: 386-480. MW: 12.1 kDa. Formulation: (Request formulation change), 50 mM HEPES, pH 8.0, with 150 mM sodium chloride, 10% glycerol. UniProt Accession #: Q04206.