Myosin, Fast Skeletal , Recombinant, Human, aa1-169, His-tag (A1 Catalytic, A2 Catalytic, Alkali Myo

Myosin, Fast Skeletal , Recombinant, Human, aa1-169, His-tag (A1 Catalytic, A2 Catalytic, Alkali Myo
Item number Size Datasheet Manual SDS Delivery time Quantity Price
M9848-05R.50 50 µg - -

1 - 19 business days

519.00€
 
Myosin regulatory light chains, including MRCL3, MYLPF and MYL9, regulate contraction in smooth... more
Product information "Myosin, Fast Skeletal , Recombinant, Human, aa1-169, His-tag (A1 Catalytic, A2 Catalytic, Alkali Myo"
Myosin regulatory light chains, including MRCL3, MYLPF and MYL9, regulate contraction in smooth muscle and non-muscle cells via phosphorylation by myosin light chain kinase (MLCK). Phosphorylation of myosin regulatory light chains, catalyzed by MLCK in the presence of calcium and calmodulin, increases the actin-activated myosin ATPase activity, thereby regulating the contractile activity. MYLPF is critically important for fast and slow skeletal muscle development. Purified by using conventional chromatography techniques. Source: Recombinant corresponding to aa1-169 of human MYLPF, fused to His-tag at N-terminus, expressed in E.coli. AA Sequence: MGSSHHHHHH SSGLVPRGSH MAPKRAKRRT VEGGSSSVFS MFDQTQIQEF KEAFTVIDQN RDGIIDKEDL RDTFAAMGRL NVKNEELDAM MKEASGPINF TVFLTMFGEK LKGADPEDVI TGAFKVLDPE GKGTIKKKFL EELLTTQCDR FSQEEIKNMW AAFPPDVGGN VDYKNICYVI , THGDAKDQE, Molecular Weight: ~21.2kD (189aa), confirmed by MALDI-TOF, Enzyme Activity: ATPase activity not determined. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Keywords: MLC2B, Fast skeletal myosin light chain 2, Myosin regulatory light chain 2, skeletal muscle isoform
Supplier: United States Biological
Supplier-Nr: M9848-05R

Properties

Conjugate: No
MW: 21,2 kD
Format: Purified

Handling & Safety

Storage: -20°C
Shipping: +4°C (International: +4°C)
Caution
Our products are for laboratory research use only: Not for administration to humans!
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