Labeled Tubulin-Biotin-XX

Source: Bovine Brain Material Labeled Tubulin-Biotin-XX is generated by reacting Biotin-XX NHS Ester with Cycled Tubulin (Cat. No. PUR-032005), thereby covalently linking the dye to random tubulin surface lysines. Cycled Tubulin is >99% pure and polymerization competent. These properties are maintained during the labeling process by reacting Cycled Tubulin with the reactive biotin in its polymerized form and subjecting it to a final polymerization/depolymerization cycle. The final product demonstrates an affinity toward streptavidin-coated beads and streptavidin-coated coverslips. Labeling stoichiometries are indicated on product labels. The product is cryopreserved at 20 mg/ml in 50 mM K-Glutamate and 0.5 mM MgCl2 (pH=7.0). Activity When supplemented with guanosine (GTP or GMPCPP) and warmed to 37°C, Labeled Tubulin-Biotin-XX will polymerize into MTs when above its critical concentration. It is recommended to combine Labeled Tubulin-Biotin-XX with unlabeled tubulin at a ratio of 1:50 to a total tubulin concentration of 2 mg/ml. We highly recommended that Cycled Tubulin (Cat. No. PUR-032005) be used as the unlabeled tubulin source to minimize tubulin heterogeneity, as labeled tubulin products are derived directly from Cycled Tubulin. Uses Labeled Tubulin-Biotin-XX is supplied for use in a variety of experimental systems including: optical traps and molecular tweezers single molecule kinesin motor assays tubulin affinity columns in vitro nanoscale devices