Labeled Tubulin-Alexa Fluor(R) 488

Item number Size Datasheet Manual SDS Delivery time Quantity Price
PUR-048805-0.1 100 µg -

4 business days*

PUR-048805-0.5 500 µg -

4 business days*

PUR-048805-1.0 1 mg -

4 business days*

Source: Bovine Brain Material Labeled Tubulin-Alexa Fluor(R) 488 is generated by reacting Alexa... more
Product information "Labeled Tubulin-Alexa Fluor(R) 488"
Source: Bovine Brain Material Labeled Tubulin-Alexa Fluor(R) 488 is generated by reacting Alexa Fluor(R) 488 NHS Ester (Thermo Fisher A20100) with Cycled Tubulin(TM) (Cat. No. PUR-032005), thereby covalently linking the dye to random tubulin surface lysines. Cycled Tubulin(TM) is >99% pure and polymerization competent. These properties are maintained during the labeling process by reacting Cycled Tubulin(TM) with the dye in its polymerized form and subjecting it to a final polymerization/depolymerization cycle. The final product displays maximum absorbance at 494 nm and a labeling stoichiometry ([dye]/[tubulin]) of 0.5-1.5 as determined by spectroscopic analysis. The product is cryopreserved at 20 mg/ml in 50 mM K-Glutamate and 0.5 mM MgCl2 (pH=7.0). Activity When supplemented with guanosine (GTP or GMPCPP) and warmed to 37°C, Labeled Tubulin-Alexa Fluor(R) 488 will polymerize into MTs when above its critical concentration. It is recommended to combine Labeled Tubulin-Alexa Fluor(R) 488 with unlabeled tubulin at a ratio of 1:9 to a total tubulin concentration of 2 mg/ml. Cycled Tubulin (Cat. No. PUR-032005) is highly recommended as the unlabeled tubulin source in order to minimize tubulin heterogeneity, as labeled tubulin products are derived directly from Cycled Tubulin. Uses Labeled Tubulin-Alexa Fluor(R) 488 is supplied for use in fluorescent experimental systems including: in vitro MT gliding assays and single molecule motor assays speckle microscopy and live cell injection in vitro nanoscale devices
Supplier: PurSolutions
Supplier-Nr: 048805


Conjugate: Alexa Fluor 488
Expressed in: Bovine
Origin: Bovine
Format: Cryopreserved

Database Information

KEGG ID : K07374 | Find alternatives
UniProt ID : P81947 | Find alternatives

Handling & Safety

Storage: -80°C (protect from light)
Shipping: -80°C (International: -80°C)
Our products are for laboratory research use only: Not for administration to humans!
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