HA-Ubiquitin-vinyl sulfone (human) (rec.) (HA)

HA-Ubiquitin-vinyl sulfone (human) (rec.) (HA)
Item number Size Datasheet Manual SDS Delivery time Quantity Price
SBB-PS0122-C050 50 µg - -

3 - 9 business days*

420.00€
 
Protein. Human ubiquitin (aa1-76) (Accession Nr. P0CG47) with a C-terminal vinyl sulfone group... more
Product information "HA-Ubiquitin-vinyl sulfone (human) (rec.) (HA)"
Protein. Human ubiquitin (aa1-76) (Accession Nr. P0CG47) with a C-terminal vinyl sulfone group and a N-terminal HA-Tag. Source: E. coli. Liquid. In 50mM MES pH 6.0, 100mM sodium chloride. Purity: >97% (LCMS). Ubiquitin is a small (8.5kDa) regulatory protein that has been found in almost all tissues of eukaryotic organisms. The addition of ubiquitin to a substrate protein is called ubiquitination or ubiquitylation. Ubiquitination can affect proteins in many ways: it can signal for their degradation via the proteasome, alter their cellular location, affect their activity and promote or prevent protein interactions. Removal of ubiquitin from a substrate protein occurs via deconjugating enzymes, of which there are nearly 100 known enzymes with various linkage specificities. This product consists of a fulllength human, mature ubiquitin polypeptide (amino acids 1-76), expressed in E.coli with a C-terminal warhead (Vinyl Sulfone) and an N-terminal HA-Tag. HA-Ubiquitin vinyl sulfone is a potent, irreversible and specific inhibitor of deubiquitinating enzymes (DUBs) based on a C-terminal electrophilic vinyl sulfone group. It targets four of the five major DUB families: UCH (Ubiquitin c-terminal hydrolases), USP (Ubiquitin Specific Proteases), OTU (Ovarian Tumor Proteases), and MJD (Machado-Josephin domain proteases) while JAMM metalloproteases are not inhibited. HA-Ubiquitin vinyl sulfone can be used for activity profiling experiments and using the HA-Tag conveniently for Immunoprecipitations in search for new or novel enzymes reacting with the c-terminal warhead.
Keywords: HA-Ub-VS, HA-Ubiquitin-VS, HA-Ubiquitin-Vinyl sulfone
Supplier: South Bay Bio
Supplier-Nr: PS0122

Properties

Conjugate: No
Host: E. coli
Species reactivity: human
MW: 10 kD
Purity: >97% (LCMS)
Format: Liquid

Handling & Safety

Storage: -80°C
Shipping: -20°C (International: -20°C)
Caution
Our products are for laboratory research use only: Not for administration to humans!
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