G9a-like protein (human recombinant)

Post-translational modification of histones by methylation of lysine residues can promote transcriptional activation or repression. G9a-like protein (GLP) and G9a are SET domain-containing methyltransferases that specifically mono- and di-methylate histone H3 at lysine 9 (H3K9). Methylation of H3K9 by a GLP/G9a heteromeric complex regulates gene expression by silencing euchromatin. GLP and G9a share 80% sequence identity in their SET domains. Structure-activity relationship (SAR) studies have been employed to design potent and selective inhibitors of these proteins, including BIX-01294 and UNC0638, with IC50 values in the nanomolar range.Synonyms: EHMT1, Euchromatic Histone-Lysine N-Methyltransferase 1, Eu HMTase 1, GLP, KMT1D. Purity: >95% estimated by SDS-PAGE. Source: active recombinant N-terminal GST-tagged protein expressed in E. coli. MW: 60.3 kDa. UniProt Accession #: Q9H9B1.