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Source: Bovine Brain Material Cycled Tubulin(TM) was isolated by cycling bovine brain homogenate through conditions that promote tubulin polymerization/depolymerization in high salt buffers by an adaptation of the method of Castoldi and Popov (2003). The resulting product is >99% pure and polymerization competent. Cycled Tubulin(TM) is supplied at 20 mg/ml as a frozen liquid in Tubulin PEM Buffer (Cat. No. PUR-032002, 80 mM PIPES, 1 mM EGTA, and 1 mM MgCl2, pH 6.8). Activity When supplemented with guanosine (GTP or GMPCPP) and warmed to 37°C, Cycled Tubulin(TM) will polymerize into MTs when above its critical concentration. The recommended tubulin concentration for ensuring polymerization is 2 mg/ml. Uses Cycled Tubulin(TM) is supplied for use in cell-free experimental systems including: structural analysis by X-ray crystallography and electron microscopy drug discovery by high-throughput screening in vitro biochemical and biophysical approaches