Chitinase domain-containing protein 1 (CHID1), human, recombinant

Organism: Homo sapiens (Human). Source: Yeast. Expression Region: 20-393aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: TLSKSDAKKA ASKTLLEKSQ FSDKPVQDRG LVVTDLKAES VVLEHRSYCS AKARDRHFAG DVLGYVTPWN SHGYDVTKVF GSKFTQISPV WLQLKRRGRE MFEVTGLHDV DQGWMRAVRK HAKGLHIVPR LLFEDWTYDD FRNVLDSEDE IEELSKTVVQ VAKNQHFDGF VVEVWNQLLS QKRVGLIHML THLAEALHQA RLLALLVIPP AITPGTDQLG MFTHKEFEQL APVLDGFSLM TYDYSTAHQP GPNAPLSWVR ACVQVLDPKS KWRSKILLGL NFYGMDYATS KDAREPVVGA RYIQTLKDHR PRMVWDSQAS EHFFEYKKSR SGRHVVFYPT LKSLQVRLEL ARELGVGVSI WELGQGLDYF YDLL. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Saccharide- and LPS-binding protein with possible roles in pathogen sensing and endotoxin neutralization. Ligand-binding specificity relates to the length of the oligosaccharides, with preference for chitotetraose (in vitro). Reference: "Structure of human stabilin-1 interacting chitinase-like protein (SI-CLP) reveals a saccharide-binding cleft with lower sugar-binding selectivity."Meng G., Zhao Y., Bai X., Liu Y., Green T.J., Luo M., Zheng X.J. Biol. Chem. 285:39898-39904(2010). Function: Saccharide- and LPS-binding protein with possible roles in pathogen sensing and endotoxin neutralization. Ligand-binding specificity relates to the length of the oligosaccharides, with preference for chitotetraose (in vitro).