Annexin V-iFluor(TM) 633 conjugate

Ex: 638 nm. Em: 655 nm. Soluble in: Water. Annexins are a family of proteins that bind to phospholipid membranes in the presence of calcium. Annexin V is a valuable tool for studying cell apoptosis. It is used as a probe to detect cells which have expressed phosphatidylserine on the cell surface, a feature found in apoptosis as well as other forms of cell death. There are a variety of parameters that can be used for monitoring cell viability. Annexin V-dye conjugates are widely used to monitor cell apoptosis through measuring the translocation of phosphatidylserine (PS). In apoptosis, PS is transferred to the outer leaflet of the plasma membrane. The appearance of phosphatidylserine on the cell surface is a universal indicator of the initial/intermediate stages of cell apoptosis and can be detected before morphological changes can be observed. iFluor 633-Annexin V conjugate has spectral properties similar to Alexa Fluor(R) 633 with fluorescence excitation and emission maxima of ~638 nm and ~655 nm respectively (Alexa Fluor(R) is the trademark of Invitrogen).