mFluor(TM) 450-VAD-FMK

mFluor(TM) 450-VAD-FMK
Item number Size Datasheet Manual SDS Delivery time Quantity Price
ABD-13475 25 tests -

3 - 8 business days*

Ex: 403 nm. Em: 454 nm. Soluble in: DMSO. FAM-VAD is a blue fluorescent cell-permeable... more
Product information "mFluor(TM) 450-VAD-FMK"
Ex: 403 nm. Em: 454 nm. Soluble in: DMSO. FAM-VAD is a blue fluorescent cell-permeable polycaspase inhibitor to target caspases 1, 2, 3, 6, 8, 9, or 10. Once inside the cell, the inhibitor binds covalently to the active caspase, which produces a green fluorescence. When added to a population of cells, the FAM-VAD-FMK probe enters each cell and covalently binds to a reactive cysteine residue that resides on the large subunit of the active caspase heterodimer, thereby inhibiting further enzymatic activity. The bound labeled reagent is retained within the cell, while any unbound reagent will diffuse out of the cell and is washed away. The green fluorescent signal is a direct measure of the amount of active caspase present in the cell at the time the reagent was added. Cells that contain the bound labeled reagent can be analyzed by 96-well plate-based fluorometry, fluorescence microscopy, or flow cytometry. The probe has the spectral properties similar to Pacific Blue® and BD HorizonT V450, thus the filter set of Pacific Blue® and BD HorizonT V450 can be conveniently used with a flow cytometer or microscope. This probe is particularly designed for the multicolor detection of caspases with a violet laser-equipped flow cytomer.
Supplier: AAT Bioquest
Supplier-Nr: 13475


Application: Caspase inhibitor, IF, FC
Conjugate: No
MW: 555,94 D

Database Information

Handling & Safety

Storage: -20°C
Shipping: +20°C (International: +20°C)
Our products are for laboratory research use only: Not for administration to humans!
Information about the product reference will follow. more
You will get a certificate here
or to request a certificate of analysis.
Read, write and discuss reviews... more
Customer review for "mFluor(TM) 450-VAD-FMK"
Write a review
or to review a product.