Tau [pT231] Negative Control Peptide (Tau Protein, Microtubule-associated Protein, MAP)

Tau [pT231] Negative Control Peptide (Tau Protein, Microtubule-associated Protein, MAP) corresponding to rabbit anti-Tau T1041-04A and is part of a kit of Positive and Negative control peptides, T1041-04. Tau proteins are important in the assembly of microtubules. In normal brain, tau is localized within the axons of the neurons, but in some neuropathological lesions, it accumulates within the body of the neuron. Tau proteins are thought to be abnormally phosphorylated in certain disease states. Tau has been immunohistochemically localized to neurofibrillary tangles of Alzheimer's disease (AD) brains. Tau proteins promote the assembly of tubulin monomers into microtubules and stabilize microtubules. Alternate splicing of tau mRNA, glycosylation, and differential phosphorylation contribute to the heterogeneity of tau. , Peptide Content: 80 ± 4%/80 ± 4%, , Solubility/Reconstitution: We recommend that each vial be briefly centrifuged prior to opening to bring the contents to the bottom. To reconstitute the peptides, pipette 100ul sterile, distilled H2O into each vial. The peptides may be gently vortexed or triturated at RT to ensure complete solubilization. If the peptides are found to be slow to dissolve, the pH of the solutions may be neutralized by addition of small volumes (1-2ul) 1.0 N ammonium hydroxide, alternatively, the peptides may be solubilized by addition of small volumes of organic solvent, such as ethanol or DMSO. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C or colder. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.