SRC1 (Steroid Receptor Coactivator 1, NCOA1, Nuclear Receptor Coactivator 1, NCoA-1, F-SRC-1) Blocki

SRC1 (Steroid Receptor Coactivator 1, NCOA1, Nuclear Receptor Coactivator 1, NCoA-1, F-SRC-1) Blocki
Item number Size Datasheet Manual SDS Delivery time Quantity Price
S6505-02A.100 100 µg - -

1 - 19 business days

221.00€
 
Peptide is used to block SRC-1 Rabbit mAb reactivity in immunohistochemistry protocols.||There... more
Product information "SRC1 (Steroid Receptor Coactivator 1, NCOA1, Nuclear Receptor Coactivator 1, NCoA-1, F-SRC-1) Blocki"
Peptide is used to block SRC-1 Rabbit mAb reactivity in immunohistochemistry protocols. There are three members of the steroid receptor co-activator (SRC) family of proteins: SRC-1 (NCoA1), SRC-2 (TIF2/GRIP1/NCoA-2) and SRC-3 (ACTR/pCIP/ RAC3/TRAM-1/AIB1). The SRC family members all share significant structural homology and function in a similar fashion to stimulate transcription mediated by nuclear hormone receptors and other transcriptional activators such as STAT3, NF-kappaB, E2F1 and p53 (1-4). Two SRC proteins, SRC-1 and SRC-3, function as histone acetyltransferases (5,6). In addition, all three family members can recruit other histone acetyltransferases (CBP/p300, PCAF) and histone methyltransferases (PRMT1, CARM1) to target promoters and cooperate to enhance expression of many genes (5-8). The SRC proteins play important roles in multiple physiological processes including cell proliferation, cell survival, somatic cell growth, mammary gland development, female reproductive function and vasoprotection (9). SRC-1 and SRC-3 are conduits for kinase-mediated growth factor signaling to the estrogen receptor and other transcriptional activators. Seven SRC-1 phosphorylation sites and six SRC-3 phosphorylation sites have been identified, which are induced by steroids, cytokines and growth factors and involve multiple kinase signaling pathways (9-11). All three SRC family members are associated with increased activity of nuclear receptors in breast, prostate and ovarian carcinomas. In addition, SRC-3 is frequently amplified or over-expressed in a number of cancers (12), and SRC-1/ PAX3 and SRC-2/MYST3 translocations are found associated with rhabdomyosarcoma and acute myeloid leukemia, respectively (13,14). , Quality Control: The quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry. The peptide blocks SRC-1 Rabbit mAb by immunohistochemistry. Directions for Use: For immunohistochemistry, add twice the volume of peptide as volume of antibody used in 100ul total volume. Incubate for a minimum of 30 minutes prior to adding the entire volume to the slide. Recommended antibody dilutions can be found on the relevant product data sheet. , Storage and Stability:, May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Supplier: United States Biological
Supplier-Nr: S6505-02A

Properties

Application: IHC, Neutr.
Conjugate: No
Format: Highly Purified

Database Information

Handling & Safety

Storage: -20°C
Shipping: +4°C (International: +4°C)
Caution
Our products are for laboratory research use only: Not for administration to humans!
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