PH Domain Leucine-rich Repeat Protein Phosphatase Blocking Peptide

To be used in conjunction with Cayman's PHLPP polyclonal antibody (Catalog No. 10007191) to block protein-antibody complex formation during immunochemical analysis of PHLPP · Protein kinase B/Akt is critical in regulating cell growth and death. Phosphorylation of a serine residue (Ser473) in a C-terminal hydrophobic motif of Akt is known to be linked to some of the most common human cancers. PHLPP dephosphorylates the hydrophobic motif of Akt and thus reduces Akt activity, resulting in a increase in the number of apoptotic cells. PHLPP levels are markedly reduced in several colon cancer and glioblastoma cell lines that have elevated Akt phosphorylation. Injection of a human glioblastoma cell line overexpressing PHLPP into mice reduced tumor size by nearly 70% compared with control animals. mRNA of PHLPP is ubiquitously expressed, with highest levels found in brain.Synonyms: KIAA0606 Protein, PHLPP, PLEKHE1 Protein, SCOP, Suprachiasmatic Nucleus Circadian Oscillatory Protein. Amino Acids: PH Domain Leucine-rich Repeat Protein Phosphatase (PHLPP) amino acids 1192-1205 (YQLDQLPDYYDTPL). Formulation: (Request formulation change), 200 µg of peptide in 200 µl TBS, pH 7.4, with 0.1% BSA and 0.02% sodium azide.