IRAK M, Blocking Peptide (IL-1 Receptor-associated Kinase), Purified

Peptide (CSSKFSWDEYEQYKKE) corresponding to amino acids 581 to 596 of human IRAK-M (3). Interleukin-1 (IL-1) and lipopolysaccharide (LPS) induces cellular response through IL-1 receptor (IL-1R) and Toll like receptors (TLR). IL-1 receptor-associated kinase (IRAK and IRAK2) mediates the activation of NF-kB by IL-1/Toll receptors (1,2), which is a pivotal transcription factor mediating in?ammatory and immune response. A novel member in the IRAK/Pelle family was recently identi?ed and designated IRAK-M(3). IRAKs associate with IL-1/Toll receptors after IL-1 or LPS stimulation and the dominant negative mutants of IRAKs inhibit IL-1 or LPS induced NF-kB activation. Members in IRAK/Pelle family play a central role in IL-1R/TLR mediated in?ammatory responses to cytokine IL-1 and LPS. , Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. , , Storage and Stability: Lyophilized powder may be stored at -20°C. Reconstitute to nominal volume by adding sterile 25% glycerol and store at -20°C. May be stored at 4°C for short-term only. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.