Interleukin-33, active (IL33), Recombinant, Human

Cytokine that binds to and signals through the IL1RL1/ST2 receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells (PubMed:16286016). Involved in the maturation of Th2 cells inducing the secretion of T-helper type 2-associated cytokines. Also involved in activation of mast cells, basophils, eosinophils and natural killer cells. Acts as a chemoattractant for Th2 cells, and may function as an "alarmin", that amplifies immune responses during tissue injury (PubMed:17853410, PubMed:18836528). {ECO:0000269, PubMed:16286016, ECO:0000269, PubMed:17853410, ECO:0000269, PubMed:18836528}., In quiescent endothelia the uncleaved form is constitutively and abundantly expressed, and acts as a chromatin-associated nuclear factor with transcriptional repressor properties, it may sequester nuclear NF-kappaB/RELA, lowering expression of its targets (PubMed:21734074). This form is rapidely lost upon angiogenic or proinflammatory activation (PubMed:18787100). {ECO:0000269, PubMed:18787100, ECO:0000269, PubMed:21734074}. Biological Activity: Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine D10S cells is less than 0.05ng/ml, corresponding to a specific activity of ? 2.0x10e7 IU/mg. Sequence: SITGISPIT EYLASLSTYN DQSITFALED ESYEIYVEDL KKDEKKDKVLLSYYESQHPS NE SGDGVDGK MLMVTLSPTK DFWLHANNKE HSVELHKCEKPLPDQAFFVL HNMHSNCVSF ECKTDPGVFI GVKDNHLALI KVDSSENLCT ENILFKLSET, Molecular Weight: 17.9kD, PubMed ID: 12819012, 10566975, 16286016, 21454686, 14702039, 15164053, 15489334, 22307629, 17853410, 17185418, 18787100, 18836528, 19596270, 19559631, 19465481, 19439663, 21734074, 22215666, 19836339, 23980170, Gene Name: IL33,C9orf26,IL1F11,NFHEV, Swiss Prot: O95760, Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.