Interleukin-16/IL-16 Protein (recombinant human)

Activity: Measured by its to chemoattract human CD4+ T-lymphocytes using a concentration range of 10.0-100.0 ng/mlThe ED50 for this effect is typically 0.1 ug/mL. Protein Construction: Recombinant Human Interleukin-16 is produced by our E.coli expression system and the target gene encoding Met1-Ser130 is expressed. Sequence: Met 1-Ser130. Endotoxin: < 1.0 EU per µg as determined by the LAL method. Apparent Molecular Mass: 18 kDa. Protein function: Interleukin-16 (IL-16) is a CD8+ T cell-derived cytokine that induces chemotaxis of CD4+ T cells and CD4+ monocytes and eosinophils. Analysis by gel filtration suggests that, under physiological conditions, human IL-16 exists predominantly as a noncovalently linked multimer, but that some IL-16 may exist as a monomer. However, only the multimeric form appears to possess chemotactic activity, suggesting that receptor cross-linking may be required for activity. IL-16 also induces expression of IL-2 receptor (IL-2R) and MHC class II molecules on CD4+ T cells. Human and murine IL-16 show significant cross-species reactivity. Protein function: Interleukin-16 stimulates a migratory response in CD4+ lymphocytes, monocytes, and eosinophils. Primes CD4+ T-cells for IL-2 and IL-15 responsiveness. Also induces T-lymphocyte expression of interleukin 2 receptor. Ligand for CD4. [The UniProt Consortium]