Insulin-like Growth Factor I, active (IGF1), Recombinant, Human

The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. May play a role in synapse maturation. {ECO:0000269, PubMed:21076856, ECO:0000269, PubMed:24132240}. Biological Activity: Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using serum free human MCF-7 cells is less than 2ng/ml, corresponding to a specific activity of ? 5.0x10e5 IU/mg. Sequence: TLCGAELVDA LQFVCGDRGF YFNKPTGYGS SSRRAPQTGI VDECCFRSCD LRRLEMYCAP LKPAKSA, Molecular Weight: 7.4kD, PubMed ID: 6358902, 3002851, 2935423, 2937782, 3455760, 2082190, 2018498, 1372070, 8495408, 16541075, 15489334, 6382022, 632300, 3242681, 21076856, 24132240, 6189745, 2036417, 1319992, 8857020, 10e391209, Gene Name: IGF1,IBP1, Swiss Prot: P05019, Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.