Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF) Recombinant, Human

Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF) Recombinant, Human
Item number Size Datasheet Manual SDS Delivery time Quantity Price
155028.10 10 µg - -

3 - 19 business days*

305.00€
155028.50 50 µg - -

3 - 19 business days*

468.00€
155028.200 200 µg - -

3 - 19 business days*

692.00€
 
Recombinant protein corresponding to aa21-160 with an N-Terminal His-Tag from human HBEF,... more
Product information "Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF) Recombinant, Human"
Recombinant protein corresponding to aa21-160 with an N-Terminal His-Tag from human HBEF, expressed in E. coli (Q99075), Amino Acid Sequence: MGHHHHHHSGSEF-VTGESLERLR RGLAAGTSNP DPPTVSTDQL LPLGGGRDRK VRDLQEADLD LLRVTLSSKP QALATPNKEE HGKRKKKGKG LGKKRDPCLR KYKDFCIHGE CKYVKELRAP SCICHPGYHG ERCHGLSLPV ENRLYTYDHT, Predicted Molecular Weight: 17.2kD, Predicted Isoelectric Point: 9.3, Subcellular Location: Secreted, extracellular space. Cell membrane, Single-pass type 1 membrane protein, Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation and SDS-PAGE., Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Included Protein Molecular Weight Marker: 168631: Unstained Protein Molecular Weight Marker, 10-70kD, Suppled as a liquid in 62.5mM Tris-H3PO4, pH 7.5, 1mM EDTA, 2% SDS, 100mM DTT, 1mM sodium azide, 0.01% bromo-phenol blue, 33% glycerol. Ready to use, no need to heat, dilute or add reducing agents before use. Protein Bands: 10kD, 14kD, 18kD, 22kD, 26kD, 33kD, 44kD and 70kD. Double Intesity Bands: The 26kD, 18kD and 10kD bands are at double intensity to make location and size approximation of proteins easier. Usage: Allow the marker to reach room temperature and mix thoroughly before use to ensure that any solids that may have precipitated at -20°C will go back into solution. Do not boil! Load the following volumes on SDS-PAGE gel: Mini gels: 3-5ul/well, Large gel: 7ul/well, The loading volumes listed above are recommended for gels with thickness of 0.75-1mm. The loading volume should be doubled for 1.5mm thick gels., Store at -20°C Stable for 6 months after receipt. Storage and Stability: Lyophilized powder may be stored at -70°C. Stable for 6 months after receipt. Reconstitute with sterile ddh2o. Aliquot to avoid repeated freezing and thawing. Store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Note: Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37°C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.
Supplier: United States Biological
Supplier-Nr: 155028

Properties

Conjugate: No
Format: Highly Purified

Database Information

UniProt ID : Q99075 | Find alternatives

Handling & Safety

Storage: vT
Shipping: +4°C (International: °C)
Caution
Our products are for laboratory research use only: Not for administration to humans!
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