Bone Morphogenetic Protein 7 (BMP7, Osteogenic Protein-1, OP-1), mouse recombinant (rmBMP7)

Bone morphogenetic protein 7 (BMP­7), also known as osteogenic protein 1 (OP­1), is a widely expressed TGF­beta super family member with important functions during embryogenesis, in the adult, and in disease. Mouse BMP­7 is synthesized with a 29aa signal sequence, a 262 aa propeptide, and a 139 aa growth fact or domain. The growth factor domain of mouse BMP­7 shares 98% and 100% aa sequence identity with human and rat BMP­7, respectively. The BMP­7 propeptide is cleaved intracellularly but remains in association with the growth factor domain. BMP­7 is subsequently secreted as a tetramer that consists of two propeptides and two disulfide­linked growth factor domains. Mature BMP­7 can also form disulfide­linked heterodimers with BMP­2 or BMP­4, complexes that show increased potency and range of activity compared to BMP­7 homodimers. The presence of the propeptides in the BMP­7 tetramer does not diminish the bioactivity of the growth factor domains. Secreted BMP­7 is immobilized in the extracellular matrix as a result of interactions between the propeptide and matrix fibrillin. BMP­7 exerts its biological effects through the type 2 receptors Activin RIIA, Activin RIIB, and BMPR­I I and the t ype 1 receptors Activin RIA, BMPR­IA, and BMPR­IB. BMP­7 plays a role in a variety of organ systems. It promotes new bone formation and nephron development, inhibits the branching of prostate epithelium, and antagonizes epithelial­mesenchymal transition (EMT). In pathological conditions, BMP­7 inhibits tumor growth and metastasis, ameliorates fibrotic damage in nephritis and promotes neuroregeneration following brain ischemia. Structure: Disulfide-linked homodimer , SDS-PAGE: 16-20kD under reducing conditions, Activity: Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED50 for this effect is typically 0.125 0.5ug/ml. Endotoxin: <1.0 EU/ug (LAL) Applications:, Suitable for use in ELISA. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with 4mM HCl. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.