Aminoacyl tRNA synthase complex-interacting multifunctional, active 1, active (AIMP1), Recombinant,

Non-catalytic component of the multisynthase complex. Stimulates the catalytic activity of cytoplasmic arginyl-tRNA synthase. Binds tRNA. Possesses inflammatory cytokine activity. Negatively regulates TGF-beta signaling through stabilization of SMURF2 by binding to SMURF2 and inhibiting its SMAD7-mediated degradation. Involved in glucose homeostasis through induction of glucagon secretion at low glucose levels. Promotes dermal fibroblast proliferation and wound repair. Regulates KDELR1-mediated retention of HSP90B1/gp96 in the endoplasmic reticulum. Plays a role in angiogenesis by inducing endothelial cell migration at low concentrations and endothelian cell apoptosis at high concentrations. Induces maturation of dendritic cells and monocyte cell adhesion. Modulates endothelial cell responses by degrading HIF-1A through interaction with PSMA7. {ECO:0000269, PubMed:10e358004, ECO:0000269, PubMed:11157763, ECO:0000269, PubMed:11306575, ECO:0000269, PubMed:11818442, ECO:0000269, PubMed:12237313, ECO:0000269, PubMed:19362550}. Biological Activity: Fully biologically active when compared to standard. The ED50 as determined by the apoptotic effect using serum free human MCF-7 cells is less than 40ng/ml, corresponding to a specific activity of ? 2.5x10e4 IU/mg. Sequence: SKPIDVSRLD LRIGCIITAR KHPDADSLYV EEVDVGEIAP RTVVSGLVNH VPLEQMQNRM VILLCNLKPA KMRGVLSQAM VMCASSPEKI EILAPPNGSV PGDRITFDAF PGEPDKELNP KKKIWEQIQP DLHTNDECVA TYKGVPFEVK GKGVCRAQTM SNSGIK, Molecular Weight: 18.2kD, PubMed ID: 7929199, 14702039, 15489334, 10e358004, 10850427, 11306575, 12237313, 11818442, 14500886, 16472771, 17443684, 19362550, 21092922, 20068231, 21269460, 22223895, 22814378, 24275569, 10852899, 11157763, Gene Name: AIMP1,EMAP2,SCYE1, Swiss Prot: Q12904, Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.