Protein Kinase C, primers, amplimer 542bp

Contents:, PCR primers for use in the detection and analysis of human mRNA transcript. This PCR-Amplimer set amplifies a specific gene transcript of size 542bp. The amplimer set includes 5' and 3' primers in separate tubes compatible with regular PCR reagents. Each set (each primer in a 50ul volume) is sufficient for at least 100 reactions. Primer A: Upstream Primer Primer B: Downstream Primer, Optimal PCR Conditions: Number of cycles: 35, Denaturation temperature: 94°C for 1 min., Annealing temperature: 55°C for 1 min., Extension temperature: 72°C for 1 min. Storage and Stability: Store at -20°C in a constant-temperature freezer.