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PCR primers for use in the detection and analysis of human mRNA transcript. This PCR-Amplimer set amplifies a specific gene transcript of size 324bp. The amplimer set includes 5' and 3' primers in separate tubes compatible with regular PCR reagents. Each set (each primer in a 50ul volume) is sufficient for at least 100 reactions. Kit Components: P9103-16Q1: Primer A, Upstream Primer 1x50ul, P9103-16Q2: Primer B, Downstream Primer 1x50ul, Optimal PCR Conditions: Number of cycles: 35, Denaturation temperature: 94°C for 1 minute, Annealing temperature: 55°C for 1 minute, Extension temperature: 72°C for 1 minute, Storage and Stability: Store at -20°C