Primers

Primers are short oligonucleotides, that usually consist of 20 to 40 nucleotides. In molecular biology, primers are mostly used for PCR (Polymerase Chain Reaction), that allows the amplification of DNA in vitro. The amplification is achieved by DNA-polymerases, to which the primers serve as starting points during DNA-synthesis. Primers can be used to obtain specific DNA-fragments for cloning experiments or, in the form of qRT-PCR (quantitative Real-Time PCR), for quantitative detection of nucleic acids.

Primers are short oligonucleotides, that usually consist of 20 to 40 nucleotides . In molecular biology, primers are mostly used for PCR (Polymerase Chain Reaction), that allows the... read more »
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Primers

Primers are short oligonucleotides, that usually consist of 20 to 40 nucleotides. In molecular biology, primers are mostly used for PCR (Polymerase Chain Reaction), that allows the amplification of DNA in vitro. The amplification is achieved by DNA-polymerases, to which the primers serve as starting points during DNA-synthesis. Primers can be used to obtain specific DNA-fragments for cloning experiments or, in the form of qRT-PCR (quantitative Real-Time PCR), for quantitative detection of nucleic acids.

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IDO1 Primer Mix-qPCR Assay
IDO1 Primer Mix-qPCR Assay

Item number: BPS-71272

Primer mix for amplifying human IDO1 cDNA (1212 bp) using dye-based, real time, quantitative PCR (RT-qPCR).
Keywords: IDO, IDO-1, Indoleamine 2,3-dioxygenase 1, Indoleamine-pyrrole 2,3-dioxygenase,
Application: RT-qPCR, RT-PCR
Species reactivity: human
208.00€ *
Review
Biotoxis qPCR Detection Kit
Biotoxis qPCR Detection Kit

Item number: Cay28322-1

Biotoxis is a qPCR all-in-one detection kit that detects B. anthracis, Y. pestis and F. tularensis. These 3 pathogens are the first potential biological weapon that can cause lethal infection. This kit is the only one on the market that detects in one reaction all 3 agents, allowing a rapid response in a context of...
Application: Sensitive B. anthracis, Y. pestis and F. tularensis multiplex qPCR detection
4,380.00€ *
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Custom Human PCR Primer Sets
Custom Human PCR Primer Sets

Item number: CHPS-1

Please inform Biomol about the Unigene Symbol of Accession Number of the corresponding gene. Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl...
Keywords: PCR, gene expression analysis
Application: RNA quantification
43.00€ *
Review
African Swine Fever Detection Kit (VP72/CD2V/MGF-PCR)
African Swine Fever Detection Kit (VP72/CD2V/MGF-PCR)

Item number: E-AD-P002.100

Tested Sample Types: Tissue, Serum, Whole Blood, Bacterial culture medium. Sensitivity: 0.994. Specificity: 0.998. Assay Time: 15mins. Result Type: Qualitative. Inter cv: <10%. Intra cv: <5%.
Application: PCR
Species reactivity: swine
From 446.00€ *
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Swine streptococcsis Detection Kit (PCR)
Swine streptococcsis Detection Kit (PCR)

Item number: E-AD-P003.100

Tested Sample Types: Swine lung, tonsil, Lymph nodes and spleen tissue samples, Stool, stillbirth and aborted fetuses of spleen and lung of serum and hydrothorax. Sensitivity: 0.995. Specificity: 0.997. Assay Time: 20mins. Result Type: Qualitative. Inter cv: <10%. Intra cv: <5%.
Application: PCR
Species reactivity: swine
From 446.00€ *
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Peanut Allergen Detection Kit (PCR)
Peanut Allergen Detection Kit (PCR)

Item number: E-FS-P003.25

Tested Sample Types: Cake, Candy, Ice cream. Assay Time: 1805s. Result Type: Qualitative. Inter cv: <10%. Intra cv: <5%.
Application: PCR
Species reactivity: broad
From 792.00€ *
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Salmonella Detection Kit (PCR)
Salmonella Detection Kit (PCR)

Item number: E-FS-P004.25

Tested Sample Types: Milk, Milk powder, Feed, Water, other food, etc. Assay Time: 3155s. Result Type: Qualitative. Inter cv: <10%. Intra cv: <5%.
Application: PCR
Species reactivity: broad
From 990.00€ *
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Oligo (dT)18 primer mix
Oligo (dT)18 primer mix

Item number: NZY-MB12801

Oligo (dT)18 primer is a synthetic single-stranded 18-mer oligonucleotide with 5'- and 3'- ends. Oligo (dT)18 is suitable for use as a primer for first-strand cDNA synthesis with a reverse transcriptase. The primer hybridizes to the poly(A) tail found at the 3'-end of most eukaryotic mRNAs, making them more specific...
Application: Cloning, cDNA library construction, qPCR
69.00€ *
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Random hexamer mix
Random hexamer mix

Item number: NZY-MB12901

Random hexamer mix includes oligonucleotides representing all possible hexamer sequences. Random hexamers are commonly used for priming singlestranded DNA or RNA for extension by DNA polymerase or Reverse transcriptase, typically when the template is difficult to copy in its entirely. During cDNA synthesis, random...
Application: cDNA synthesis, RT-PCR, RT-qPCR
57.00€ *
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Gapdh, Mouse Glyceraldehyde phosphate dehydrogenase - GAPD, Real Time PCR Primer Set
Gapdh, Mouse Glyceraldehyde phosphate dehydrogenase -...

Item number: VMPS-7317

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template. Recommended...
Keywords: Gapdh, GAPDH, mCG_134295, EC=1.2.1.12, Glyceraldehyde-3-phosphate dehydrogenase
Application: RNA quantification
43.00€ *
Review
Mouse Housekeeping Genes Primer Sets
Mouse Housekeeping Genes Primer Sets

Item number: MHK-1

Includes primer sets for ACTB, B2M, GAPD, GUSB, HPRT1, PGK, PP1A, and RPL13A. Normalize real time PCR results to multiple housekeepers rather than one for more accurate quantitation. Perform up to 1000 assays (based on 20 µl assay volume) for each housekeeping gene.
Application: RT-PCR, Gene expression analysis
121.00€ *
Review
Mouse ER Stress (UPR) Primer Library
Mouse ER Stress (UPR) Primer Library

Item number: MEPR-I

Keywords: MEPR-I
Species reactivity: mouse
854.00€ *
Review
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