UGQuencher-2 NHS ester [equivalent to BHQ 2 NHS ester]

UGQuencher-2 NHS ester, which is equivalent to BHQ 2 NHS ester, is a dark quencher featuring a polyaromatic-azo structure that prevents any intrinsic fluorescence. This non-emissive quencher is widely used in qPCR probes and other fluorescence-quenched probe applications. Its broad and strong absorption range (560-670 nm) makes it highly effective as an acceptor in FRET applications, particularly when paired with orange to far-red emitting dyes such as Cy3, Cy5, ROX, Texas Red, Alexa Fluor(R) 546, 555, 594, 633, 647, and IRDye(R) 650. The NHS ester (or succinimidyl ester) group is a commonly used amine-reactive functionality, enabling covalent conjugation to primary amines in proteins (e.g., lysine residues), amine-modified oligonucleotides, and other amine-containing molecules. Labeling is most efficient under mildly alkaline conditions (pH 7-9), resulting in the formation of stable amide bonds. This combination of properties makes UGQuencher-2 NHS ester a preferred choice for developing fluorescence-quenched probes and biomolecular conjugates. Chemical Properties Storage Upon receipt, store at -20°C in the dark. Protect from light and moisture. When stored as indicated, UGQuencher-2 NHS ester is stable for 3 years. This product is for Research Use Only· Not for Human or Veterinary or Therapeutic Use CAS number 916753-62-3 Formula C29H29N7O8 Molecular Weight 603.59 Purity >95%