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Ex: 573 nm. Em: 588 nm. Soluble in: DMSO. Cal-590(TM) has been developed to improve Rhod-2 cell loading and calcium response while maintaining the spectral wavelength of Rhod-2, making it compatible with TRITC/Cy3(R) filter set. In CHO and HEK cells Cal-590(TM) AM has cellular calcium response that is much more sensitive than Rhod-2 AM. The spectra of Cal-590 is well separated from those of FITC, Alexa Fluor(R) 488 and GFP, making it an ideal calcium probe for multiplexing intracellular assays with GFP cell lines or FITC/Alexa Fluor(R) 488 labeled antibodies.