Cal-520(TM), sodium salt

Cal-520(TM), sodium salt
Item number Size Datasheet Manual SDS Delivery time Quantity Price
ABD-21135 500 µg (10 x 50 µg) -

3 - 8 business days*

ABD-21136 1 mg -

3 - 8 business days*

Cal-520(TM) provides a robust homogeneous fluorescence-based assay tool for detecting... more
Product information "Cal-520(TM), sodium salt"
Cal-520(TM) provides a robust homogeneous fluorescence-based assay tool for detecting intracellular calcium mobilization. Cal-520(TM) AM is a new fluorogenic calcium-sensitive dye with a significantly improved signal to noise ratio and intracellular retention compared to the existing green calcium indicators (such as Fluo-3 AM and Fluo-4 AM). Cells expressing a GPCR or calcium channel of interest that signals through calcium can be preloaded with Cal-520(TM) AM which can cross cell membrane. Once inside the cell, the lipophilic blocking groups of Cal 520(TM) AM are cleaved by esterases, resulting in a negatively charged fluorescent dye that stays inside cells. Its fluorescence is greatly enhanced upon binding to calcium. When cells stimulated with agonists, the receptor signals the release of intracellular calcium, which significantly increase the fluorescence of Cal-520(TM). The characteristics of its long wavelength, high sensitivity, and >100 times fluorescence enhancement, make Cal-520(TM) AM an ideal indicator for the measurement of cellular calcium. The high S/N ratio and better intracellular retention make the Cal-520(TM) calcium assay a robust tool for evaluating GPCR and calcium channel targets as well as for screening their agonists and antagonists.
Supplier: AAT Bioquest
Supplier-Nr: 21135


Application: Calcium detection

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Handling & Safety

Storage: -20°C
Shipping: +20°C (International: +20°C)
Our products are for laboratory research use only: Not for administration to humans!
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