Dyes and Labeling Reagents

This category comprises chemicals for permanent and temporary labeling of organelles, proteins, and other molecules. For example, this includes the covalent linkage of a target protein, such as an antibody, with a fluorescent dye/fluorophore, allowing detection of the target protein in microscopic experiments. For the purpose of detection, proteins can also be coupled with reporter enzymes (e.g. HRP) or radioactive isotopes. To facilitate the purification of a target protein, biotinylation followed by streptavidine affinity binding has proven to be effective.

This category comprises chemicals for permanent and temporary labeling of organelles, proteins, and other molecules. For example, this includes the covalent linkage of a target protein, such as an... read more »
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Dyes and Labeling Reagents

This category comprises chemicals for permanent and temporary labeling of organelles, proteins, and other molecules. For example, this includes the covalent linkage of a target protein, such as an antibody, with a fluorescent dye/fluorophore, allowing detection of the target protein in microscopic experiments. For the purpose of detection, proteins can also be coupled with reporter enzymes (e.g. HRP) or radioactive isotopes. To facilitate the purification of a target protein, biotinylation followed by streptavidine affinity binding has proven to be effective.

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Hoechst 33342 Stain, 1 ml
Hoechst 33342 Stain, 1 ml

Item number: ICT-639

Cell-permeant nuclear counterstain that emits blue fluorescence when bound to dsDNA.
Application: Nuclear counterstain, Cell cycle studies
33.00€ *
Review
Calcein AM
Calcein AM

Item number: Cay14948-1

Calcein AM is a cell-permeable dye that, upon entering live cells, is cleaved by intracellular esterases, leaving membrane-impermeant calcein (Cay-16221), a fluorescent indicator with absorption and emission maxima of 494 and 517 nm, respectively. It can be used as an indicator of cell viability, cell-cell...
Keywords: Calcein Acetoxymethyl ester, NSC 689290,...
Application: Cell-permeable dye, cell viability assays
CAS 148504-34-1
MW: 994.9 D
From 98.00€ *
Review
Phalloidin-iFluor(TM) 488 Conjugate
Phalloidin-iFluor(TM) 488 Conjugate

Item number: ABD-23115

This green fluorescent phalloidin conjugate (equivalent to Alexa Fluor(R) 488-labeled phalloidin) selectively binds to F-actins with much higher photostability than the fluorescein-phalloidin conjugates. Used at nanomolar concentrations, phalloidin derivatives are convenient probes for labeling, identifying and...
Application: Labeling
MW: 1,9 kD
208.00€ *
Review
Phalloidin-iFluor(TM) 647 Conjugate
Phalloidin-iFluor(TM) 647 Conjugate

Item number: ABD-23127

This deep red fluorescent phalloidin conjugate (equivalent to Alexa Fluor(R) 633-labeled phalloidin) selectively binds to F-actins. Used at nanomolar concentrations, phalloidin derivatives are convenient probes for labeling, identifying and quantitating F-actins in formaldehyde-fixed and permeabilized tissue...
Application: Labeling
MW: 2,20 kD
208.00€ *
Review
FMOC-Lys(5-FITC)-OH
FMOC-Lys(5-FITC)-OH

Item number: ABD-5070

A building block for in-sequence Lys labeling by 5-FITC.
Application: Building block
CAS 1095493-05-2
279.00€ *
Review
FDP (Fluorescein diphosphate, tetraammonium salt)
FDP (Fluorescein diphosphate, tetraammonium salt)

Item number: ABD-11600

Upon interaction with phosphatases the colorless and non-fluorescent FDP is hydrolyzed to highly fluorescent fluorescein. FDP has been widely used in various ELISA assays. Additionally it is also used to detect tyrosine phosphatases.
Application: Phosphatase substrate
CAS 217305-49-2
512.00€ *
Review
2-NBDG [2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose]
2-NBDG...

Item number: ABD-36702

Ex: 485 nm. Em: 540 nm. Soluble in: DMSO. 2-NBDG is a fluorescently-labeled deoxyglucose analog that is used primarily to directly monitor glucose uptake by living cells and tissues. It is also used as a topical contrast reagent for the detection of neoplasia. 2-NBDG can be used in real-time confocal,...
Application: IF, FC, glucose uptake monitoring
CAS 186689-07-6
MW: 342,26 D
208.00€ *
Review
RatioWorks(TM) PDMPO
RatioWorks(TM) PDMPO

Item number: ABD-21204

The existing pH probes are ill-adapted to study acidic organelles such as lysosomes, endosomes, spermatozoa and acrosomes because their fluorescence is significantly reduced at lower pH. In addition, most of the existing pH probes (such as BCECF and SNARF) are not selectively localized in acidic organelles. The...
Application: Fluorescent pH indicator
139.00€ *
Review
DiBAC4(3) (Bis-(1,3-dibutylbarbituric acid)trimethine oxonol)
DiBAC4(3) (Bis-(1,3-dibutylbarbituric acid)trimethine...

Item number: ABD-21411

DiBAC4(5) is a sensitive membrane potential probe. Slow-response probes are suitable for detecting changes in average membrane potentials of nonexcitable cells.
Application: Membrane potential probe
CAS 70363-83-6
139.00€ *
Review
Cal-520(TM), potassium salt
Cal-520(TM), potassium salt

Item number: ABD-21140

Cal-520(TM) provides a robust homogeneous fluorescence-based assay tool for detecting intracellular calcium mobilization. Cal-520(TM) AM is a new fluorogenic calcium-sensitive dye with a significantly improved signal to noise ratio and intracellular retention compared to the existing green calcium indicators (such...
Application: Calcium detection
From 418.00€ *
Review
Fura Red, AM
Fura Red, AM

Item number: ABD-21048

Ex: 458 nm. Em: 597 nm. Soluble in: DMSO. Fura Red is a visible light-excitable fura-2 analog that offers unique possibilities for ratiometric measurement of calcium ion in single cells by microphotometry, imaging or flow cytometry when used with single excitation, green-fluorescent calcium indicators. Fura Red AM...
Application: Cell-permeable ratiometric fluorescent calcium indicator, Fura-2 analog, FC, Single cell imaging
CAS 149732-62-7
MW: 1089 D
From 348.00€ *
Review
EGTA AM
EGTA AM

Item number: ABD-21005

EGTA AM is cell-permeable version of EGTA, a cell-permeable calcium chelator.
Application: Calcium chelator
CAS 99590-86-0
208.00€ *
Review
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