Biological Buffers

Most chemical reactions are severely influenced by pH changes of the solution. Biological buffers allow the researcher to maintain control of the solution’s acidity by capturing or releasing protons. The pH value is kept constant even after addition of acids of bases. This is of special importance with regards to biochemical reactions, as the charge of proteins and nucleic acids is strongly correlated to the local pH. Biological solutions like blood or saliva are therefore buffered, mostly by phosphate- or carbonate buffer systems. In molecular biology and biochemistry, buffering is achieved by addition of so-called “good buffers” or PBS.

Most chemical reactions are severely influenced by pH changes of the solution. Biological buffers allow the researcher to maintain control of the solution’s acidity by capturing or releasing... read more »
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Biological Buffers

Most chemical reactions are severely influenced by pH changes of the solution. Biological buffers allow the researcher to maintain control of the solution’s acidity by capturing or releasing protons. The pH value is kept constant even after addition of acids of bases. This is of special importance with regards to biochemical reactions, as the charge of proteins and nucleic acids is strongly correlated to the local pH. Biological solutions like blood or saliva are therefore buffered, mostly by phosphate- or carbonate buffer systems. In molecular biology and biochemistry, buffering is achieved by addition of so-called “good buffers” or PBS.

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MES Free Acid (2-(N-Morpholino) ethanesulfonic acid)
MES Free Acid (2-(N-Morpholino) ethanesulfonic acid)

Item number: M3000.250

MES Free Acid is a biological buffer (zwitterionic) designed by Good, et al., and typically referred to as Good's buffer useful in cell culture media formulations. Selection of biological buffer systems should include the following criteria: exclusion by biological membranes, low absorption between 240 and 700nm,...
CAS 4432-31-9
MW: 195.20 D
From 341.00€ *
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TES, ultra pure
TES, ultra pure

Item number: 22080.100

Useful pH range: 7.0-8.0
CAS 7365-44-8
MW: 229.18
Please request pricing for this article.
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Tris Base USP (Tris(hydroxymethyl) aminomethane,_2-Amino-2-(hydro xymethyl)-1, 3-propanediol,_Tromet
Tris Base USP (Tris(hydroxymethyl)...

Item number: T8601.1

Tris is one of the most widely used buffers in molecular biology and cell culture due to its low toxicity, stability and buffering capacity. Our USP Grade is very similar in specifications to ultrapure Tris (T8600). Purity: >99.0%, Appearance: White, crystalline powder, Solubility (1M): Colorless, clear,...
CAS 77-86-1
MW: 121.14 D
From 61.00€ *
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MES, Sodium Salt (2-(N-Morpholino) ethanesulfonic acid sodium salt)
MES, Sodium Salt (2-(N-Morpholino) ethanesulfonic acid...

Item number: M3005.100

MES Sodium Salt is a biological buffer (zwitterionic) designed by Good et al., and typically referred to as Good's buffer. It is useful in cell culture media formulations. Selection of biological buffer systems should include the following criteria: exclusion by biological membranes, low absorption between...
CAS 71119-23-8
MW: 217.20 D
From 142.00€ *
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HEPES Sodium Salt (N-[2-Hydroxyethyl] piperazine-N'- [2-ethanesulfonic acid] sodium salt)
HEPES Sodium Salt (N-[2-Hydroxyethyl] piperazine-N'-...

Item number: H2030.500

HEPES Sodium is a biological buffer (zwitterionic) designed by Good, et al., and typically referred to as Good's buffer useful in cell culture media formulations. Selection of biological buffer systems should include the following criteria: exclusion by biological membranes, low absorption between 240 and 700nm,...
CAS 75277-39-3
MW: 260.30 D
From 144.00€ *
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Tris Acetate (Tris(hydroxymethyl) aminomethane acetate, 2-Amino-2-(hydroxymethyl)-1, 3-propanediol a
Tris Acetate (Tris(hydroxymethyl) aminomethane acetate,...

Item number: T8600-05.100

Tris acetate is used for the production of TAE buffer for molecular biology applications. Appearance: White, crystalline powder, Purity: >99%, Melting Point: 115-125°C, Solubility (1%, dH2O): Colorless, clear, complete , pH (1%, dH2O): 6.0-7.0, Absorbance: 250nm: <0.3, 260nm: <0.1, 280nm: <0.05, Chloride: <0.05%,...
CAS 6850-28-8
MW: 181.20 D
From 70.00€ *
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Tricine (N-[tris-hydroxymethyl]methyllglycine)
Tricine (N-[tris-hydroxymethyl]methyllglycine)

Item number: T8400.250

Tricine is a biological buffer (zwitterionic) designed by Good, et al., and typically referred to as Good's buffer useful in cell culture media formulations. Selection of biological buffer systems should include the following criteria: exclusion by biological membranes, low absorption between 240 and 700nm,...
CAS 5704-04-1
MW: 179.20 D
From 190.00€ *
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Phosphate Buffer, APHA, pH 7.2 (Butterfield's Buffered Phosphate Diluent) (Powder)
Phosphate Buffer, APHA, pH 7.2 (Butterfield's Buffered...

Item number: P4071-50.100

Intended Use:, Phosphate Buffer, pH 7.2 is used for the preparation of microbiological dilution blanks. Product Summary: The formula for phosphate buffer is specified by the American Public Health Association (APHA) for use in diluting test samples. Phosphate Buffer, pH 7.2 is specified for use in diluting water,...
From 199.00€ *
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PIPES Free Acid (Piperazine-N,N'-bis(2-ethane sulfonic acid))
PIPES Free Acid (Piperazine-N,N'-bis(2-ethane sulfonic...

Item number: P4200.250

PIPES is a biological buffer (zwitterionic) designed by Good, et al., and typically referred to as Good's buffer useful in cell culture media formulations. Selection of biological buffer systems should include the following criteria: exclusion by biological membranes, low absorption between 240-700nm, chemically...
CAS 5625-37-6
MW: 302.40 D
From 119.00€ *
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TAPSO Free Acid (3-[N-tris-(hydroxymethyl) methylamino]-2- hydroxypropanesulfonic acid)
TAPSO Free Acid (3-[N-tris-(hydroxymethyl)...

Item number: T1010.1

TAPSO Free Acid is a biological buffer (zwitterionic) designed by Good, et al., and typically referred to as Good's buffer useful in cell culture media formulations. Selection of biological buffer systems should include the following criteria: exclusion by biological membranes, low absorption between 240 and 700nm,...
CAS 68399-81-5
MW: 259.30 D
From 106.00€ *
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NP-40 Cell Lysis Buffer (Mild)
NP-40 Cell Lysis Buffer (Mild)

Item number: BPS-82124-1

The NP-40 Cell Lysis Buffer is intended for the lysis of mammalian adherent and suspension-cultured cells. This buffer is suitable for extraction of cytoplasmic proteins in mild, non-denaturing conditions.
From 52.00€ *
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Modified NP-40 Cell Lysis Buffer (Mild)
Modified NP-40 Cell Lysis Buffer (Mild)

Item number: BPS-82125-1

The Modified NP-40 Cell Lysis Buffer is intended for the lysis of mammalian adherent and suspension-cultured cells. This buffer is stronger than the standard NP-40 Cell Lysis Buffer.
From 67.00€ *
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