o-Nitrophenyl beta-D-Galactopyranoside

o-Nitrophenyl beta-D-galactopyranoside (ONPG) is a chromogenic substrate for beta-galactosidase. Upon enzymatic cleavage by beta-galactosidase, o-nitrophenol is released, which can be quantified by colorimetric detection at 420 nm as a measure of beta-galactosidase activity.Formal Name: 2-nitrophenyl beta-D-galactopyranoside. CAS Number: 369-07-3. Synonyms: ortho-Nitrophenyl beta-D-galactopyranoside, o-Nitrophenyl beta-D-Galactoside, ortho-Nitrophenyl beta-D-Galactoside, NSC 83631, ONPG. Molecular Formula: C12H15NO8. Formula Weight: 301.3. Purity: >98%. Formulation: (Request formulation change), A crystalline solid. Solubility: DMF: 20 mg/ml, DMSO: 15 mg/ml, Ethanol: 0.25 mg/ml, PBS (pH 7.2): 2 mg/ml. lambdamax: 212, 256, 310 nm. SMILES: OC[C@H]1O[C@@H](OC2=C([N+]([O-])=O)C=CC=C2)[C@H](O)[C@@H](O)[C@H]1O. InChi Code: InChI=1S/C12H15NO8/c14-5-8-9(15)10(16)11(17)12(21-8)20-7-4-2-1-3-6(7)13(18)19/h1-4,8-12,14-17H,5H2/t8-,9+,10+,11-,12-/m1/s1. InChi Key: KUWPCJHYPSUOFW-YBXAARCKSA-N.