Nuclear Yellow

Nuclear yellow is a DNA-selective dye that is used to label DNA content in live and fixed cells. Upon binding to DNA, nuclear yellow fluoresces, and this fluorescence can be measured using fluorescence microscopy, microplate fluorometry, or flow cytometry. Nuclear yellow has commonly been used in combination with retrograde tracers for two-color neuronal mapping. It can also be used to photoconvert diaminobenzidine (DAB) into an electron-dense reaction product for light and electron microscopy applications. Nuclear yellow displays excitation/emission maxima of 355/495 nm, respectively.Formal Name: 4-[5-(4-methyl-1-piperazinyl)[2,5'-bi-1H-benzimidazol]-2'-yl]-benzenesulfonamide, trihydrochloride. CAS Number: 74681-68-8. Synonyms: Hoechst S769121. Molecular Formula: C25H25N7O2S . 3HCl. Formula Weight: 597.0. Emission: 495 nm. Excitation: 355 nm. Formulation: (Request formulation change), A powder. Solubility: DMSO: Soluble, Water: Soluble. SMILES: CN1CCN(C2=CC(N=C(C3=CC(N=C(C4=CC=C(S(N)(=O)=O)C=C4)N5)=C5C=C3)N6)=C6C=C2)CC1.Cl.Cl.Cl. InChi Code: InChI=1S/C25H25N7O2S.3ClH/c1-31-10-12-32(13-11-31)18-5-9-21-23(15-18)30-25(28-21)17-4-8-20-22(14-17)29-24(27-20)16-2-6-19(7-3-16)35(26,33)34,,,/h2-9,14-15H,10-13H2,1H3,(H,27,29)(H,28,30)(H2,26,33,34),3*1H. InChi Key: NZVGXJAQIQJIOY-UHFFFAOYSA-N.