DYn-2

Mild oxidation can convert the sulfhydryl group of cysteine residues on proteins to cysteine-sulfenic acid derivatives (Cys-SOH). Protein sulfenylation, then, is a post-translational modification that is relevant to redox signaling. DYn-2 is a chemoselective probe for detecting sulfenylated proteins in intact cells. DYn-2 consists of 1,3-cyclohexanedione coupled to an alkyne moiety by a 3-carbon spacer. The cyclohexanedione group selectively reacts with protein sulfenic acid modifications. The alkyne group of DYn-2 can then be detected using azide-bearing tags by standard click chemistry. This approach offers superior sensitivity relative to using azide-modified probes with alkynyl detection tags.Formal Name: 4-(pent-4-yn-1-yl)cyclohexane-1,3-dione. CAS Number: 1354630-46-8. Synonyms: Click Tag(TM) DYn-2. Molecular Formula: C11H14O2. Formula Weight: 178.2. Purity: >98%. Formulation: (Request formulation change), A crystalline solid. Solubility: DMF: 30 mg/ml, DMSO: 20 mg/ml, Ethanol: 20 mg/ml. lambdamax: 255 nm. SMILES: O=C1CCC(CCCC#C)C(C1)=O. InChi Code: InChI=1S/C11H14O2/c1-2-3-4-5-9-6-7-10(12)8-11(9)13/h1,9H,3-8H2. InChi Key: UNMICBPHYXLMRA-UHFFFAOYSA-N. License: Sold under license from The Scripps Research Institute..