BAPTA (free acid)

White to off-white powder Soluble in DMSO (20mg/ml) or DMF (20mg/ml). Chemical. CAS: 85233-19-8. Formula: C22H24N2O10. MW: 476.4. BAPTA is a membrane-impermeable highly selective calcium chelator and is useful for manipulating extracellular Ca2+ levels, with 105-fold greater affinity for Ca2+ than for Mg2+. The presence of four carboxylic acid functional groups allows for the binding of two calcium ions. BAPTA and its derivatives can be used as calcium indicators, since the absorption maximum for BAPTA changes when it is complexed with calcium (absorption maxima free/complexed = 254/274 nm, emission maxima free/complexed = 363/363 nm). Key advantages of BAPTA include relative insensitivity toward intracellular pH change and fast release of calcium. This product is a high quality and sensitive compound for calcium signaling studies, investigation of signal transduction, apoptotic cascades and neuroscience research.