Aminopropargyl dCTP [5-Propargylamino-2'-deoxycytidine-5'-triphosphate]

Aminopropargyl dCTP [5-Propargylamino-2'-deoxycytidine-5'-triphosphate]
Item number Size Datasheet Manual SDS Delivery time Quantity Price
ABD-17050 10 µmol -

3 - 8 business days*

580.00€
 
The amine-modified deoxycytidine 5'-triphosphates (such as... more
Product information "Aminopropargyl dCTP [5-Propargylamino-2'-deoxycytidine-5'-triphosphate]"
The amine-modified deoxycytidine 5'-triphosphates (such as 5-propargylamino-2'-deoxycytidine-5'-triphosphate) can be used to produce amine-containing DNA by conventional enzymatic incorporation methods such as reverse transcription, nick translation, random primed labeling, or PCR. Aminopropargyl dCTP can be readily incorporated into DNA through the conventional enzymatic incorporation techniques. The resulting amine-modified nucleic acids can then be labeled using any of amine-reactive fluorescent dyes, biotins and other amine-reactive reagents. The aminopropargyl-modified nucleotides can be incorporated to extremely high and consistent levels compared to the tag-labeled nucleotides that generally have higher stereo-hindrance. Subsequent reaction of the amine-modified nucleic acid with an excess of amine-reactive reagent achieves correspondingly high and consistent labeling efficiencies, regardless of the labeling reagent chosen. This two-step labeling method also eliminates the need to optimize an enzymatic reaction to accommodate different dye-modified nucleotides, which may incorporate at very different rates. This labeling method is widely used for both FISH probes and microarray-based experiments
Supplier: AAT Bioquest
Supplier-Nr: 17050

Properties

Application: Two-step DNA labelling, FISH, Microarrays
MW: 608,15 D
Format: Desiccated

Database Information

Handling & Safety

Storage: -20°C (protect from light)
Shipping: -20°C (International: -20°C)
Signal Word: Warning
P Phrases: P304, P312
Caution
Our products are for laboratory research use only: Not for administration to humans!
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