FCGR2A (Human) CRISPR/Cas9 Lentivirus (Non-Integrating)

Item number Size Datasheet Manual SDS Delivery time Quantity Price
BPS-78199 1 ml (500 µl x 2) - -

3 - 15 business days*

FCGR2A1 (Low Affinity IIa Receptor (FcgammaRIIa), also known as CD32a) encodes one member of a... more
Product information "FCGR2A (Human) CRISPR/Cas9 Lentivirus (Non-Integrating)"
FCGR2A1 (Low Affinity IIa Receptor (FcgammaRIIa), also known as CD32a) encodes one member of a family of immunoglobulin Fc receptor genes found on the surface of many immune response cells such as macrophages and neutrophils. It is involved in the process of phagocytosis and clearing immune complexes. FCGR2A is involved in activating cellular responses, while FCGR2B is inhibitory, and the balance between these receptors regulates immune activation and tolerance. The FCGR2A CRISPR Lentiviruses are replication incompetent, HIV-based VSV-G pseudotyped lentiviral particles that are ready to infect almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a CRISPR/Cas9 gene driven by an EF1a promoter, along with 5 sgRNA (single guide RNA) targeting human FCGR2A driven by a U6 promoter (Figures 1 and 2). Unlike FCGR2A CRISPR/Cas9 Lentivirus (Integrating) (BPS Bioscience, #78207), the FCGR2A (human) CRISPR/Cas9 Lentivirus (Non-Integrating) is made with a mutated Integrase, therefore the viral DNA cannot integrate into the genome, resulting in only transient expression of Cas9 and CD32a-targeting sgRNA. It is expected that this will minimize potential off-target effects caused by either prolonged expression or random integration of Cas9 and the sgRNA. A short round of puromycin selection right after transduction may increase knockout efficiency, however puromycin should not be used for more than 48 hours post-transduction due to the transient nature of expression using the non-integrating lentivirus.  Note:  Efficiencies may vary, depending on the cell type and the gene of interest.
Keywords: CD32, CDw32, FCGR2A, FcRII-a, Fc-gamma-RIIa, Fc-gamma RII-a, IgG Fc receptor II-a, Low affinity immunoglobulin gamma Fc region receptor II-a,
Supplier: BPS Bioscience
Supplier-Nr: 78199


Application: CRISPR studies
Reactivity: Human

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