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Total RNA was extracted and purified from cells using RNA isolation & purification kit according to manufacturers instructions. The RNA was diluted to 200 ng/µL in RNase free water. Concentration was determined at A260 using nanodrop ND-1000. Total RNA was prepared from cell line WM1791C. WM1791C is a metastatic human melanoma cell line. This cell line features the wild type KRAS and KRAST501, KRASE62K mutation. This cell line also expresses a K22Q mutation in CDK4. The K22Q mutation results in an amino acid substitution at position 22 in CDK4, from a lysine (K) to a glutamine (Q). This cell line is wild type for BRAF, and c-KIT genes. WM1791C cells produce xenograft tumors when injected into immunocompromised mice.
This website uses cookies, which are necessary for the technical operation of the website and are always set. Other cookies, which increase the usability of this website, serve for direct advertising or simplify interaction with other websites and social networks, will only be used with your consent.
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