MRC-5 Cell Line

Background: The MRC-5 cell line was derived from normal lung tissue of a 14-week-old male fetus in 1966. The cells are capable of 42 to 46 population doublings before the onset of senescence. This is a normal diploid human cell line with 46, XY karyotype. The modal chromosome number was 46, occurring in 70% of cells. Species: Homo sapiens, Human. Age: Male,14FW. Tissue: Lung. Morphology: Fibroblast. Growth properties: Adherent. Doubling time: 36-96 hours. Biosafety: 1. Medium: MEM+10% FBS+1% P/S. Subculturing: Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Ratio: 1:2-1:4. to 2 times per week. Cryopreservation: Freeze Medium: 55% Basal Medium+40% FBS+5% DMSO Storage Temperature: Liquid Nitrogen Vapor Phase. Culture conditions: Atmosphere: Air, 95%, CO2, 5%, Temperature: 37 °C.