HET-1A Cells

Categories: Transformed cell lines Description: The HET-1A cell line is derived from the human esophageal epithelium and is used extensively in gastroenterological research. These cells provide a valuable model for studying the physiology and pathology of the esophagus, particularly in the context of esophageal diseases such as Barrett's esophagus and esophageal cancer. HET-1A cells are often employed to investigate the cellular responses to various environmental and dietary factors that may contribute to esophageal disease development and progression. HET-1A cells exhibit an epithelial morphology and retain characteristics typical of esophageal epithelial cells, including the expression of cytokeratins and other epithelial markers. They are used in studies focusing on epithelial cell biology, differentiation, and the mechanisms of cellular transformation. Researchers utilize HET-1A cells to explore the effects of acid and bile reflux, oxidative stress, and inflammation on esophageal cells, providing insights into the pathophysiology of gastroesophageal reflux disease (GERD) and its potential progression to Barrett's esophagus or esophageal adenocarcinoma. Additionally, HET-1A cells are used to assess the impact of various chemopreventive and therapeutic agents on esophageal epithelial health, making them an important tool for advancing the understanding and treatment of esophageal disorders. Organism: Human Tissue: Esophagus Synonyms: Het-1A, HET1A, Het1A Age: 74 years Gender: Male Ethnicity: African American Morphology: Epithelial Cell Type: Epithelial cell Growth Properties: Adherent Citation: HET-1A (Cytion catalog number 305270) Biosafety Level: 2 Ncbi_ Taxid: 9606.0 Cellosaurus Accession: CVCL_3702 Protein Expression: Cytokeratin Antigen Expression: SV40 T antigen Tumorigenic: No Viruses: Transformant: Simian virus 40 (SV40) Culture Medium: BEGM Bronchial Epithelial Cell Growth Medium BulletKit (from Lonza, Lonza catalog number CC-3170) Dissociation Reagent: Accutase Subculturing: Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. Fluid Renewal: 2 to 3 times per week Freeze Medium: As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress. Thawing And Culturing Cells: Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks, for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes. Sterility: Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. Safety Precautions: When planning to store a cryovial in liquid nitrogen for future thawing, it is mandatory to adhere to stringent safety measures. Appropriate protective gloves and clothing are essential, and the use of a face mask or safety goggles is required during the transfer of frozen samples to or from the liquid nitrogen tank. This is to mitigate the risk of injury from potential cryovial explosions upon removal, which can result in the projection of sharp fragments. Warranty: We stand by the promise of delivering products with high cell viability and robust culture performance. To achieve the best results, please make sure you follow the storage and culture instructions detailed in the product information sheet closely. Your adherence to these guidelines is key to success. Subject To Material Transfer Agreements: If you intend to use Cytion cell lines solely for internal research at a single research site, please complete and sign our Material Transfer Agreement (MTA) and submit it along with your order.For any commercial applications - including but not limited to fee-for-service work, quality control testing, product release, diagnostic use, or regulatory studies - please complete the Intended Use Form so we can prepare a suitable agreement tailored to your project.Please note: The MTA applies only to certain cell lines. If this notice and the MTA document appear on a product page, the agreement is applicable. For cell lines not covered by the MTA, no reference to the agreement will be shown. The MTA is not valid for customers in the Americas, China, or Taiwan. Please contact our U.S. entity to receive the appropriate agreement. Required Product 3: 860015.0 Required Product 4: 830100.0