Cells and Cell Lines

Cell culture and microbial culture techniques are essential for almost every aspect of biological research since modern microbiology started in the 19th century. The invention of microbial cultures of bacteria and yeast in sterile culture media allows selective growing of monoclonal cultures. From these cultures, competent cells can be generated for the use in transformation. Cell culture colloquially refers to cultures of animal cells, in which we differentiate between ex vivo cultures and immortalized cell lines. Ex vivo cultures are derived from blood or organs and have a limited life span. Immortalized cell lines, however, are usually derived from tumors and proliferate indefinitely due to mutations in the cell cycle controls.

Cell culture and microbial culture techniques are essential for almost every aspect of biological research since modern microbiology started in the 19th century. The invention of microbial... read more »
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Cells and Cell Lines

Cell culture and microbial culture techniques are essential for almost every aspect of biological research since modern microbiology started in the 19th century. The invention of microbial cultures of bacteria and yeast in sterile culture media allows selective growing of monoclonal cultures. From these cultures, competent cells can be generated for the use in transformation. Cell culture colloquially refers to cultures of animal cells, in which we differentiate between ex vivo cultures and immortalized cell lines. Ex vivo cultures are derived from blood or organs and have a limited life span. Immortalized cell lines, however, are usually derived from tumors and proliferate indefinitely due to mutations in the cell cycle controls.

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WM3211 Viable Cells
WM3211 Viable Cells

Item number: WM3211-01-0001

Cells are sterile, validated by short tandem repeat profiling, and are tested as negative for mycoplasma. It is recommended that cell lines are tested for mycoplasma contamination and short tandem repeat (STR) profiling every 10 passages or each time a frozen seed stock is made. See cell culture protocol for...
Application: Genetic studies, xenograft production, drug testing, drug target discovery
Host: Human
Species reactivity: human
From 1,568.00€ *
Review
Cas9 Expressing iPS Cell Pool
Cas9 Expressing iPS Cell Pool

Item number: BPS-78578

The Cas9 Expressing iPS Cell Pool constitutively expresses Cas9 (Streptococcus pyogenes CRISPR associated protein 9) under the control of an EF1A promoter, introduced into PBMC-derived human iPS cells via lentiviral transduction (Cas9 Lentivirus, BPS Bioscience #78066). Cas9 Expressing iPS cells can be transduced...
Keywords: iPSC,
Application: sgRNA-edited iPS cell pool generation, pooled sgRNA library screening (pluripotency/differentiation)
Species reactivity: human
11,185.00€ *
Review
Cas9 Inducible (Tet-On) iPS Cell Pool
Cas9 Inducible (Tet-On) iPS Cell Pool

Item number: BPS-78845

The Cas9 Inducible (Tet-On) iPS Cell Pool expresses the Cas9 (Streptococcus pyogenes CRISPR associated protein 9) gene under the control of a tight TRE tetracycline-inducible promoter, introduced into PBMC-derived human iPS cells via lentiviral transduction [Cas9 Lentivirus (inducible TET on), BPS Bioscience...
Keywords: iPSC,
Application: Temporally controlled gene editing induction, sgRNA-edited iPS cell pool generation, screening
Species reactivity: human
11,345.00€ *
Review
DLL3 CHO Cell Line
DLL3 CHO Cell Line

Item number: BPS-78882

CHO-K1 cells expressing human delta like canonical Notch ligand 3 (DLL3) gene (accession number NM_016941.3) under the control of the cytomegalovirus (CMV) promoter. This cell line was generated by lentiviral transduction followed by puromycin selection and limited dilution. Individual clones were screened based on...
Keywords: DLL3, Delta3, Delta-like protein 3, Drosophila Delta homolog 3,
Application: Validation target cells, small molecule DLL3 targeting drug validation/characterization, CAR-T study
Species reactivity: human
9,308.00€ *
Review
Claudin-3 CHO Cell Line
Claudin-3 CHO Cell Line

Item number: BPS-78566

Recombinant Claudin-3 CHO K1 cell line stably expressing the full-length human Claudin-3 (accession number: NM_001306). Surface expression of hClaudin-3 was confirmed by flow cytometry. The stable clonal cell line was selected for high level of hClaudin-3 expression compared to the parental CHO K1 cells.
Keywords: CLDN3, hRVP1, C7orf1, CPE-R 2, Claudin-3, CPE-receptor 2, Rat ventral prostate.1 protein homolog, Clostridium perfringens...
Application: Antibody-med signaling immunotherapy studies, drug discovery, screening, antib./ligand characterization
Species reactivity: human
9,308.00€ *
Review
Claudin-4 CHO Cell Line
Claudin-4 CHO Cell Line

Item number: BPS-78567

Recombinant Claudin-4 CHO K1 cell line stably expressing the full-length human Claudin-4 (accession number: NM_001305). Surface expression of hClaudin-4 was confirmed by flow cytometry. Each stable clonal cell line was selected for high level of hClaudin-4 expression compared to the parental CHO K1 cells.
Keywords: CLDN4, CPE-R, Claudin-4, CPE-receptor, Clostridium perfringens enterotoxin receptor, Williams-Beuren syndrome chromosomal...
Application: Antibody-med signaling immunotherapy studies, drug discovery, screening, antib./ligand characterization
Species reactivity: human
9,308.00€ *
Review
CRE/CREB Luciferase Reporter CHO Cell Line (cAMP/PKA Signaling Pathway)
CRE/CREB Luciferase Reporter CHO Cell Line (cAMP/PKA...

Item number: BPS-78568

The CRE/CREB Luciferase Reporter CHO Cell Line (cAMP/PKA Signaling Pathway) contains a firefly luciferase gene under the control of multimerized cAMP response element (CRE) stably integrated into CHO cells. Elevation of the intracellular cAMP level activates cAMP response element binding protein (CREB) to bind CRE...
Keywords: CREB1, CREB-1, cAMP-responsive element-binding protein 1, Cyclic AMP-responsive element-binding protein 1,
Application: cAMP/PKA signaling pathway activity monitoring, activator / inhibitor screening
Species reactivity: human
3,056.00€ *
Review
NFAT Luciferase Reporter CHO Cell Line (PKC/Ca2+ Signaling Pathway)
NFAT Luciferase Reporter CHO Cell Line (PKC/Ca2+...

Item number: BPS-78569

The NFAT Luciferase Reporter CHO cell line (PKC/Ca2+ Signaling Pathway) contains a firefly luciferase gene under the control of NFAT response element stably integrated into CHO K1 cells. This cell line is validated for its response to phorbol 12-myristate 13-acetate (PMA) in the presence of ionomycin.
Keywords: TonEBP, NF-AT5, TonE-binding protein, T-cell transcription factor NFAT5, Nuclear factor of activated T-cells 5,...
Application: NFAT stimulation monitoring, PKC/ Ca2+ activator/inhibitor screening
Species reactivity: human
3,056.00€ *
Review
B7-H4 CHO Cell Line (low Expression)
B7-H4 CHO Cell Line (low Expression)

Item number: BPS-78586-L

B7-H4 CHO Cell line is a recombinant clonal stable CHO-K1 cell line constitutively expressing full length human B7- H4 protein, also known as V-set domain-containing T-cell activation inhibitor 1 (VTCN1), (NM_024626.4). Surface expression of B7-H4 was confirmed by flow cytometry. Each stable clonal cell line was...
Keywords: B7-H4, B7h.5, Protein B7S1, B7 homolog 4, T-cell costimulatory molecule B7x, Immune costimulatory protein B7-H4, V-set...
Application: Antibody screening/validation, drug discovery, modulator/inhibitor screening (cellular context)
Species reactivity: human
9,308.00€ *
Review
eGFP/Firefly Luciferase MIA PaCa-2 Cell Line
eGFP/Firefly Luciferase MIA PaCa-2 Cell Line

Item number: BPS-78766

Recombinant MIA PaCa-2 cells expressing firefly luciferase and enhanced GFP (eGFP) driven by an EF1a promoter, generated by transduction with Firefly Luciferase-eGFP lentivirus (BPS Bioscience #78741).
Keywords: Luciferase, Luciferin 4-monooxygenase,
Application: CAR-T / NK co-culture killing assay target cells, in vitro/in vivo bioluminescence imaging (BLI)
4,544.00€ *
Review
NY-ESO-1 TCR (1G4) CD8+ NFAT-Luciferase Reporter Jurkat Cell Line
NY-ESO-1 TCR (1G4) CD8+ NFAT-Luciferase Reporter Jurkat...

Item number: BPS-78769

This cell line was generated from T Cell Receptor (TCR) Knockout NFAT Luciferase Reporter Jurkat Cell Line (BPS Bioscience #78556) by overexpression of human CD8 (NM_001768.6) and NY-ESO-1 TCR (1G4) using lentiviral transduction (CD8a Lentivirus #78648 and NY-ESO-1 -Specific TCR Lentivirus Clone 1G4 #78675). The...
Keywords: MAL, CD8A, CD8a, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2,
Application: Co-culture bioassay design/optimization (NY-ESO-1-specific TCR cell evaluation), positive control
Species reactivity: human
16,706.00€ *
Review
NY-ESO-1 TCR (c259) CD8+ NFAT-Luciferase Reporter Jurkat Cell Line
NY-ESO-1 TCR (c259) CD8+ NFAT-Luciferase Reporter Jurkat...

Item number: BPS-78771

This cell line was generated from the T Cell Receptor (TCR) Knockout NFAT Luciferase Reporter Jurkat Cell Line (BPS Bioscience #78556) by overexpression of human CD8 (NM_001768.6) and NY-ESO-1 TCR (c259) using lentiviral transduction (CD8a Lentivirus #78648 and NY-ESO-1 -Specific TCR Lentivirus Clone c259 #78676)....
Keywords: MAL, CD8A, CD8a, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2,
Application: Co-culture bioassay design/optimization (NY-ESO-1-specific TCR cell evaluation), positive control
Species reactivity: human
16,706.00€ *
Review
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