Prostaglandin E Metabolite ELISA Kit

Prostaglandin E Metabolite ELISA Kit
Item number Size Datasheet Manual SDS Delivery time Quantity Price
Cay514531-96S 96 so-wells (96 solid wells) -

6 - 10 business days*

Cay514531-96 96 st-wells (96 strip wells) -

6 - 10 business days*

Cay514531-480 480 st-wells (480 strip wells) -

6 - 10 business days*

Cay514531-480S 480 so-wells (480 solid wells) -

6 - 10 business days*

Prostaglandin E2 (PGE2) is produced by a variety of cell types which, in general, do not contain... more
Product information "Prostaglandin E Metabolite ELISA Kit"
Prostaglandin E2 (PGE2) is produced by a variety of cell types which, in general, do not contain the enzymes required for metabolism of PGE2. Thus, cultured endothelial cells or osteoblasts will release PGE2 into the culture medium, where it will accumulate without appreciable metabolism. The direct assay of PGE2 from the medium is a good way to measure PGE2 production from these cells. PGE2 is rapidly converted in vivo to its 13,14-dihydro-15-keto metabolite, with more than 90% of circulating PGE2 cleared by a single passage through the lungs. Unfortunately, this metabolite is not chemically stable and undergoes a variable amount of degradation to PGA products. For this reason, blood, urine, or other samples from whole animals or humans often contain very little intact PGE2, and measurement of the metabolites is necessary to provide a reliable estimate of actual PGE2 production. Cayman's PGE Metabolite assay was developed as a method of converting 13,14-dihydro-15-keto PGA2 and 13,14-dihydro-15-keto PGE2 to a single, stable derivative that could be easily quantified by ELISA. This assay is therefore the method of choice if the samples in question have undergone extensive metabolism prior to collection. Synonyms: PGE Metabolite EIA Kit, PGEM. Assay Range: 0.39-50 pg/ml. Sensitivity: 80% B/B0: ~2 pg/ml. Mid-point: 50% B/B0: ~8-14 pg/ml. Cross Reactivity: (13,14-dihydro-15-keto Prostaglandin E1 (derivatized)) 100%, (13,14-dihydro-15-keto Prostaglandin E2 (derivatized)) 100%, (Bicyclo Prostaglandin E1) 38%, (13,14-dihydro-15-keto Prostaglandin D2 (derivatized)) 0.08%, (13,14-dihydro-15-keto Prostaglandin F2alpha (derivatized)) 0.02%, (Arachidonic Acid) <0.01%, (Leukotriene B4) <0.01%, (tetranor-PGEM) <0.01%, (tetranor-PGFM) <0.01%, (Prostaglandin D2) <0.01%, (Prostaglandin E1) <0.01%, (6-keto Prostaglandin E1) <0.01%, (Prostaglandin E2) <0.01%, (Prostaglandin F1alpha) <0.01%, (6-keto Prostaglandin F1alpha) <0.01%, (Prostaglandin F2alpha) <0.01%, (Thromboxane B2) <0.01%. Origin: Animal/Bovine, Animal/Eel, Animal/Mouse, Animal/Rabbit.
Keywords: PGE Metabolite EIA Kit, PGEM
Supplier: Cayman Chemical
Supplier-Nr: 514531


Application: ELISA
Format: Solid Phase

Database Information

Handling & Safety

Storage: -20°C
Shipping: -20°C (International: -20°C)
Signal Word: Danger
GHS Hazard Pictograms:
H Phrases: H225, H301, H302, H311, H313, H315, H316, H317, H318, H319, H335, H351, H361
P Phrases: P201, P202, P210, P233, P240, P241, P242, P243, P261, P264, P270, P271, P272, P280, P301+310, P302+352, P303+361+353, P304+340, P305+351+338, P308+313, P310, P312, P321, P330, P332+313, P333+313, P337+313, P361+364, P362+364, P370+378, P403+233, P403+235, P405, P501
Our products are for laboratory research use only: Not for administration to humans!
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