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Prostaglandin D2 (PGD2) is biosynthesized in the brain by a soluble, 26 kDa glutathione-independent lipocalin-type PGD2 synthase. This PGD2 accumulates in the cerebrospinal fluid (CSF), where it induces physiologic sleep in rats and humans. PGD2 is also synthesized in mast cells and leukocytes by a cellular, myeloid-type, glutathione-dependent PGD synthase. This PGD2 which is formed in the intracellular and vascular compartments is rapidly metabolized to 11beta-PGF2alpha and other metabolites. Measurement of the parent eicosanoid PGD2 is appropriate in cell culture lysates and in CSF, where concentrations of several hundred pg/ml have been measured. Cayman's PGD2 ELISA Kit is a competitive assay that can be used for quantification of PGD2 in cell culture lysates and enzymatic reactions.Synonyms: PGD2 EIA Kit. Assay Range: 19.5-2,500 pg/ml. Sensitivity: 80% B/B0: 55 pg/ml. Mid-point: 50% B/B0: 170-310 pg/ml. Cross Reactivity: (Prostaglandin D2) 100%, (Prostaglandin F2alpha) 92.4%, (Prostaglandin J2) 21.6%, (Prostaglandin E2) 2.86%, (Thromboxane B2) 2.54%, (11beta-Prostaglandin F2alpha) 1.99%, (8-iso Prostaglandin F2alpha) 1.90%, (Prostaglandin A2) 0.72%, (12(S)-HHTrE) 0.16%, (6-keto Prostaglandin F1alpha) 0.05%, (13,14-dihydro-15-keto Prostaglandin D2) 0.02%, (Arachidonic Acid) <0.01%, (Leukotriene D4) <0.01%, (tetranor-PGDM) <0.01%, (tetranor-PGEM) <0.01%, (tetranor-PGFM) <0.01%, (tetranor-PGJM) <0.01%. Origin: Animal/Eel, Animal/Goat, Animal/Bovine, Animal/Mouse.
This website uses cookies, which are necessary for the technical operation of the website and are always set. Other cookies, which increase the usability of this website, serve for direct advertising or simplify interaction with other websites and social networks, will only be used with your consent.
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