Prostaglandin D2 ELISA Kit

Prostaglandin D2 (PGD2) is biosynthesized in the brain by a soluble, 26 kDa glutathione-independent lipocalin-type PGD2 synthase. This PGD2 accumulates in the cerebrospinal fluid (CSF), where it induces physiologic sleep in rats and humans. PGD2 is also synthesized in mast cells and leukocytes by a cellular, myeloid-type, glutathione-dependent PGD synthase. This PGD2 which is formed in the intracellular and vascular compartments is rapidly metabolized to 11beta-PGF2alpha and other metabolites. Measurement of the parent eicosanoid PGD2 is appropriate in cell culture lysates and in CSF, where concentrations of several hundred pg/ml have been measured. Cayman's PGD2 ELISA Kit is a competitive assay that can be used for quantification of PGD2 in cell culture lysates and enzymatic reactions.Synonyms: PGD2 EIA Kit. Assay Range: 19.5-2,500 pg/ml. Sensitivity: 80% B/B0: 55 pg/ml. Mid-point: 50% B/B0: 170-310 pg/ml. Cross Reactivity: (Prostaglandin D2) 100%, (Prostaglandin F2alpha) 92.4%, (Prostaglandin J2) 21.6%, (Prostaglandin E2) 2.86%, (Thromboxane B2) 2.54%, (11beta-Prostaglandin F2alpha) 1.99%, (8-iso Prostaglandin F2alpha) 1.90%, (Prostaglandin A2) 0.72%, (12(S)-HHTrE) 0.16%, (6-keto Prostaglandin F1alpha) 0.05%, (13,14-dihydro-15-keto Prostaglandin D2) 0.02%, (Arachidonic Acid) <0.01%, (Leukotriene D4) <0.01%, (tetranor-PGDM) <0.01%, (tetranor-PGEM) <0.01%, (tetranor-PGFM) <0.01%, (tetranor-PGJM) <0.01%. Origin: Animal/Eel, Animal/Goat, Animal/Bovine, Animal/Mouse.