PD-1 BioAssay(TM) ELISA Kit, Human

PD-1 BioAssay(TM) ELISA Kit, Human
Item number Size Datasheet Manual SDS Delivery time Quantity Price
144005.96 96 tests - -

1 - 19 business days

734.00€
 
Programmed cell death protein 1, also called PD-1 or PDCD1 is a protein that in humans is encoded... more
Product information "PD-1 BioAssay(TM) ELISA Kit, Human"
Programmed cell death protein 1, also called PD-1 or PDCD1 is a protein that in humans is encoded by the PDCD1 gene. This gene encodes a cell surface membrane protein of the immunoglobulin superfamily. By fluorescence in situ hybridization, the PDCD1 gene is mapped to 2q37.3. This protein is expressed in pro-B-cells and is thought to play a role in their differentiation. In mice, expression of this gene is induced in the thymus when anti-CD3 antibodies are injected and large numbers of thymocytes undergo apoptosis. Mice deficient for this gene bred on a BALB/c background developed dilated cardiomyopathy and died from congestive heart failure. These studies suggest that this gene product may also be important in T cell function and contribute to the prevention of autoimmune diseases. Intended Use: PD-1 BioAssay(TM) ELISA Kit is a sandwich ELISA for quantitative detection of human PD-1 in cell culture supernates. Range: 156pg/ml-10,000pg/ml, Sensitivity: <10pg/ml, Specificity: Natural and recombinant human PD-1, Crossreactivity: There is no detectable cross-reactivity with other relevant proteins. Kit Components: 144005A: Microtiter Strips, 1x96 wells, 144005B: PD-1 Standard, 2x10ng, 144005C: Anti-human PD-1 (Biotin), 1x130ul (1:100 dilution), USB Cat No Kit Component 143696: Avidin-Biotin-Peroxidase Complex (ABC), 1x130ul (1:100 dilution), 143697: Sample Diluent Buffer, 1x30ml, 143698: Antibody Diluent Buffer, 1x12ml, 143699: ABC Diluent Buffer, 1x12ml, 143700: TMB Color Developing Agent, 1x10ml, 143701: TMB Stop Solution, 2N H2SO4, 1x10ml, Storage and Stability: Store *144005B powder at 4°C. Once reconstituted store at 4°C for up to 12 hours or at -20°C for up to 48 hours. Store other components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Assay Summary: 1. Add samples and standards and incubate the plate at 37°C for 90 minutes. Do not wash. , 2. Add biotinylated antibodies and incubate the plate at 37°C for 60 minutes. Wash plate 3 times with 0.01M TBS or PBS., 3. Add ABC working solution and incubate the plate at 37°C for 30 minutes. Wash plate 5 times with 0.01M TBS or PBS., 4. Add TMB color developing agent and incubate the plate at 37°C in dark for 15-20 minutes., 5. Add TMB stop solution and read.
Keywords: PD1, PDCD1, hPD-1, CD279, Protein PD-1, Programmed cell death protein 1
Supplier: United States Biological
Supplier-Nr: 144005

Properties

Application: ELISA
Reactivity: Human
Format: Solid Phase

Handling & Safety

Storage: vT
Shipping: +4°C (International: +4°C)
Caution
Our products are for laboratory research use only: Not for administration to humans!
You will get a certificate here
or to request a certificate of analysis.
Read, write and discuss reviews... more
Customer review for "PD-1 BioAssay(TM) ELISA Kit, Human"
Write a review
or to review a product.
Information about the product reference will follow. more
Viewed