Mouse GNaO1 (G Protein Alpha Activating Activity Polypeptide O) ELISA (Small Sample Volume)

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse GNaO1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse GNaO1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse GNaO1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse GNaO1 in the samples is then determined by comparing the OD of the samples to the standard curve. Protein Function: Guanine nucleotide-binding proteins (G proteins) function as transducers downstream of G protein-coupled receptors (GPCRs) in numerous signaling cascades (PubMed:10926822). The alpha chain contains the guanine nucleotide binding site and alternates between an active, GTP-bound state and an inactive, GDP-bound state (PubMed:10926822). Signaling by an activated GPCR promotes GDP release and GTP binding (PubMed:10926822). The alpha subunit has a low GTPase activity that converts bound GTP to GDP, thereby terminating the signal (PubMed:10926822). Both GDP release and GTP hydrolysis are modulated by numerous regulatory proteins (PubMed:10926822). Signaling is mediated via effector proteins, such as adenylate cyclase (PubMed:10926822). Inhibits adenylate cyclase activity, leading to decreased intracellular cAMP levels (PubMed:10926822) [The Uniprot Consortium]