Insulin (human) ELISA Kit

Insulin (human) ELISA Kit
Item number Size Datasheet Manual SDS Delivery time Quantity Price
Cay26619-96 96 wells -

6 - 10 business days*

778.00€
 
This immunoassay is based on a sandwich technique. Wells of supplied plate are coated with a... more
Product information "Insulin (human) ELISA Kit"
This immunoassay is based on a sandwich technique. Wells of supplied plate are coated with a monoclonal antibody specific to insulin. Insulin is introduced in the wells where it is bound by the monoclonal antibody coated on the plate. Insulin is then detected by an insulin-specific monoclonal antibody tagged with acetylcholinesterase (AChE). The two antibodies thus form a sandwich by binding on different epitopes of insulin. The concentration of insulin is determined by measuring the enzymatic activity of immobilized tracer using Ellman's reagent. AchE tracer acts on Ellman's reagent to form a yellow compound that strongly absorbs at 405 nm or at 414 nm . Insulin in a polypeptide composed of 51 amino acids divided into two chains (A and B) linked by disulfide bonds. Both chains are organized as alpha helix. Insulin is produced by the beta cells of the pancreatic islets in form of a precursor (prepro-insulin) and is modified to obtain the pro-insulin (peptide signal elimination) and finally insulin (peptide C elimination). The main function of the insulin is the regulation of the glucose concentration in the organism. The dysregulation of the production of insulin induced many disorders including diabetes. [Bertin Bioreagent Product Number [A05322]]Synonyms: Insulin (human) EIA Kit. Limit of Detection: =0.3 µIU/ml (calculated as the concentration of insulin corresponding to the NSB average plus three standard deviations). Assay Range: 0.4 µIU/ml - 52 µIU/ml [15.4 pg/ml - 2,000 pg/ml]. Cross Reactivity: (100%) Insulin (pig), (Not detected) Pro-Insulin (human), (Not detected) C-Peptide (human).
Keywords: Insulin (human) EIA Kit
Supplier: Cayman Chemical
Supplier-Nr: 26619

Properties

Application: ELISA
Species reactivity: human
Format: Solid Phase

Handling & Safety

Storage: +4°C
Shipping: +4°C (International: +4°C)
Signal Word: Danger
GHS Hazard Pictograms:
H Phrases: H290, H314, H315, H318, H319
P Phrases: P234, P260, P264, P280, P310, P321, P363, P390, P301+P330+P331, P302+P352, P303+P361+P353, P304+P340, P305+P351+P338, P332+P313, P337+P313, P362+P364, P405, P406, P501
Caution
Our products are for laboratory research use only: Not for administration to humans!
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