COX (human) Inhibitor Screening Assay Kit

The COX (human) Inhibitor Screening Assay directly measures PGF2alpha by stannous chloride reduction of COX-derived PGH2 produced in the COX reaction. The prostanoid product is quantified via ELISA using an antiserum specific to PGF2a. This assay includes recombinant human COX-1 and COX-2 and is an excellent tool for general COX-1 and COX-2 inhibitor screening. It will also help to eliminate false positive leads generated by less specific methods.Synonyms: Cyclooxygenase, PGHS, Prostaglandin H Synthase. Assay Range: 3.9-500 pg/ml. Sensitivity: 10 pg/ml. Mid-point: 40-60 pg/ml. Cross Reactivity: (100%) Prostaglandin F2alpha, (244.1%) Prostaglandin F2alpha Ethanolamide, (61%) Prostaglandin F1alpha, (21%) Prostaglandin F3alpha, (5.3%) 20-hydroxy Prostaglandin F2alpha, (2.6%) Prostaglandin E2, (1.2%) Prostaglandin D2, (0.69%) 19(R)-hydroxy Prostaglandin F2alpha, (0.1%) 13,14-dihydro-15-keto Prostaglandin F2alpha, (0.03%) 11beta-Prostaglandin F2alpha, (0.02%) 6,15-diketo-13,14-dihydro Prostaglandin F1alpha, (0.01%) 11-dehydro Thromboxane B, (<0.01%) 8-iso Prostaglandin F2alpha. Origin: Animal/Rabbit, Animal/Bovine, Animal/Eel.