ADAM9, BioAssay(TM) ELISA Kit (Human) (Disintegrin and Metalloproteinase Domain-containing Protein 9

Disintegrin and metalloproteinase domain-containing protein 9, also known as MCMP or MDC9 is an enzyme that in humans is encoded by the ADAM9 gene. This gene encodes a member of the ADAM('a disintegrin and metalloproteinase') family and exhibits a number of characteristics of class III reprolysins. This gene is mapped to 8p11.22. Members of this family are membrane- anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. Sample Type: Cell culture supernates, serum and plasma (heparin), Intended Use: For quantitative detection of human ADAM9 in cell culture supernates, serum and plasma (heparin). Sensitivity: <10pg/ml, Range: 15.6-1000pg/ml, Specificity: Natural and recombinant human ADAM9, Test Principle: This human ADAM9 BioAssay(TM) ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for ADAM9 has been precoated onto 96-well plates. Standards(NSO, A29-D697) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ADAM9 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human ADAM9 amount of sample captured in plate. Kit Components: 96-well plate precoated with human ADAM9 antibody, 1, Lyophilized recombinant human ADAM9 standard, 10ng/tube×2, Biotinylated human ADAM9 antibody, 130ul (dilution 1:100), Avidin-Biotin-Peroxidase Complex (ABC), 130ul (dilution 1:100), Sample diluent buffer, 30ml, Antibody diluent buffer, 12ml, ABC diluent buffer, 12ml, TMB color developing agent, 10ml, TMB stop solution, 10ml, Storage and Stability: Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.