PPARdelta (human) Reporter Assay Kit

Item number Size Datasheet Manual SDS Delivery time Quantity Price
IB-IB00122 384 wells -

7 - 11 business days*

3,802.00€
 
This nuclear receptor assay system utilizes proprietary non-human cells engineered to provide... more
Product information "PPARdelta (human) Reporter Assay Kit"
This nuclear receptor assay system utilizes proprietary non-human cells engineered to provide constitutive, high-level expression of the human peroxisome proliferator-activated receptor delta (NR1C2), a ligand-dependent transcription factor commonly referred to as PPARD or PPARdelta. INDIGO's reporter cells include the luciferase reporter gene functionally linked to a PPARdelta-responsive promoter. Thus, quantifying changes in luciferase expression in the treated reporter cells provides a sensitive surrogate measure of the changes in PPARdelta activity. The principal application of this reporter assay system is in the screening of test samples to quantify any functional activity, either agonist or antagonist, that they may exert against human PPARdelta. PPARdelta reporter cells are prepared using INDIGO's proprietary CryoMite(TM) process. This cryo-preservation method yields exceptional cell viability post-thaw, and provides the convenience of immediately dispensing healthy, division-competent reporter cells into assay plates. There is no need for cumbersome intermediate treatment steps such as spin-and-rinse of cells, viability determinations, cell titer adjustments, or the preincubation of reporter cells prior to assay setup. INDIGO Bioscience's nuclear receptor reporter assays are all-inclusive cell-based assay systems. In addition to PPARdelta reporter cells, this kit provides two optimized media for use during cell culture and in diluting the user's test samples, a reference agonist, luciferase detection reagent, and a cell culture-ready assay plate.
Keywords: NUCI, NUC1, NR1C2, PPARD, PPAR-beta, PPAR-delta, Nuclear hormone receptor 1, Nuclear receptor subfamily 1 group C member 2, Peroxisome proliferator-activated receptor beta, Peroxisome proliferator-activated receptor delta
Supplier: Indigo Biosciences
Supplier-Nr: IB00122

Properties

Application: Functional activity screening
Species reactivity: human
Format: Solid Phase

Handling & Safety

Storage: -80°C
Shipping: -80°C (International: -80°C)
Caution
Our products are for laboratory research use only: Not for administration to humans!
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