PPARalpha (human) Reporter Assay Kit

PPARalpha (human) Reporter Assay Kit
Item number Size Datasheet Manual SDS Delivery time Quantity Price
IB-IB00111 96 wells - -

5 - 17 business days*

IB-IB00111-32 96 wells (3x32 wells) - -

5 - 17 business days*

This nuclear receptor assay system utilizes proprietary non-human cells engineered to provide... more
Product information "PPARalpha (human) Reporter Assay Kit"
This nuclear receptor assay system utilizes proprietary non-human cells engineered to provide constitutive, high-level expression of the human peroxisome proliferator-activated receptor alpha (NR1C1), a ligand-dependent transcription factor commonly referred to as PPARA or PPARalpha. INDIGO's reporter cells include the luciferase reporter gene functionally linked to a PPARalpha-responsive promoter. Thus, quantifying changes in luciferase expression in the treated reporter cells provides a sensitive surrogate measure of the changes in PPARalpha activity. The principal application of this reporter assay system is in the screening of test samples to quantify any functional activity, either agonist or antagonist, that they may exert against human PPARalpha. PPARalpha reporter cells are prepared using INDIGO's proprietary CryoMite(TM) process. This cryo-preservation method yields exceptional cell viability post-thaw, and provides the convenience of immediately dispensing healthy, division-competent reporter cells into assay plates. There is no need for cumbersome intermediate treatment steps such as spin-and-rinse of cells, viability determinations, cell titer adjustments, or the pre-incubation of reporter cells prior to assay setup. INDIGO Bioscience's nuclear receptor reporter assays are all-inclusive cell-based assay systems. In addition to PPARalpha reporter cells, this kit provides two optimized media for use during cell culture and in diluting the user's test samples, a reference agonist, luciferase detection reagent, and a cell culture-ready assay plate.
Keywords: NR1C1, PPARA, PPAR-alpha, Nuclear receptor subfamily 1 group C member 1, Peroxisome proliferator-activated receptor alpha
Supplier: Indigo Biosciences
Supplier-Nr: IB00111


Application: Functional activity screening
Reactivity: Human
Format: Solid Phase

Handling & Safety

Storage: -80°C
Shipping: -80°C (International: -80°C)
Our products are for laboratory research use only: Not for administration to humans!
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